fiber (AC_000007) Virus Tagged ORF Clone

CAT#: VC100051

Myc-DDK-tagged ORF clone of viral ORF for fiber [Human adenovirus 2], codon optimized for human cell expression, AP_000190

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: fiber
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100051 represents NCBI reference of AP_000190 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCGGGCTCGGCCCTCCGAGGATACGTTCAATCCCGTGTACCCCTACGATACCGAGACAGGACCTC
  CTACTGTGCCCTTTCTTACACCACCCTTCGTGAGTCCTAATGGGTTCCAAGAATCACCCCCGGGGGTGCT
  CTCTCTGCGGGTTAGCGAGCCCCTGGATACCAGCCATGGAATGCTCGCACTCAAGATGGGGAGTGGTCTC
  ACTCTGGATAAGGCCGGGAACCTGACATCCCAGAATGTCACAACCGTAACTCAGCCTCTGAAGAAGACTA
  AGTCTAATATCTCACTCGACACCAGCGCCCCACTGACAATTACCTCTGGAGCACTGACAGTGGCCACAAC
  CGCTCCTCTGATAGTCACTAGCGGAGCACTCTCAGTGCAGTCCCAAGCACCTCTTACCGTCCAGGACTCC
  AAGCTGTCCATAGCTACCAAAGGGCCTATTACAGTGTCCGACGGCAAGCTCGCCCTGCAGACTTCCGCGC
  CCCTGTCTGGCTCAGACTCTGACACCCTGACTGTGACCGCCTCCCCACCCTTGACTACTGCTACAGGCAG
  CCTCGGAATCAATATGGAAGATCCTATCTATGTTAACAATGGAAAAATCGGAATTAAGATCTCCGGCCCC
  TTGCAGGTAGCCCAGAATTCAGACACACTCACTGTGGTGACAGGTCCGGGCGTTACCGTCGAGCAGAATA
  GCTTGCGGACTAAGGTCGCGGGTGCAATTGGCTATGATAGCTCAAACAATATGGAAATTAAAACCGGAGG
  TGGGATGCGGATTAACAATAACCTGCTGATCCTGGATGTAGACTATCCCTTTGATGCACAGACTAAACTC
  CGACTGAAGCTGGGCCAGGGTCCACTGTACATAAATGCATCTCACAACCTCGACATTAATTACAACCGGG
  GATTGTACCTGTTCAATGCGAGCAACAATACTAAAAAGCTCGAGGTCTCTATTAAAAAGTCATCCGGACT
  GAATTTCGATAATACCGCTATCGCTATCAACGCAGGCAAAGGGCTTGAATTTGATACTAATACGAGCGAA
  TCACCCGATATCAACCCTATCAAAACGAAGATCGGTTCAGGCATCGATTACAACGAAAACGGCGCTATGA
  TTACTAAGCTCGGAGCCGGACTGTCATTTGACAATAGCGGCGCCATTACGATTGGGAATAAGAATGACGA
  TAAACTCACCCTCTGGACCACCCCAGACCCCTCTCCTAACTGTAGGATACACTCAGATAACGACTGTAAG
  TTTACCCTGGTATTGACAAAGTGTGGCAGCCAGGTACTTGCAACCGTGGCCGCTTTGGCGGTCTCCGGGG
  ACTTGTCTTCAATGACTGGAACAGTGGCCTCCGTCTCCATCTTCCTGCGATTCGATCAGAATGGGGTGTT
  GATGGAGAATTCATCTCTGAAGAAGCATTATTGGAACTTCCGAAACGGAAATTCTACCAACGCCAATCCA
  TATACTAACGCAGTCGGGTTCATGCCCAACCTCCTGGCTTACCCTAAAACACAGAGTCAGACAGCCAAGA
  ACAATATAGTCTCCCAAGTATATTTGCATGGAGACAAGACGAAGCCAATGATCCTCACTATCACACTTAA
  TGGCACTAGCGAGAGCACAGAGACTTCCGAAGTAAGCACCTACTCAATGTCATTTACCTGGTCCTGGGAA
  AGCGGCAAATACACCACAGAGACCTTTGCCACGAACTCTTATACTTTTTCTTATATCGCCCAGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100051 representing AP_000190
  Red=Cloning sites Green=Tags
  
  MKRARPSEDTFNPVYPYDTETGPPTVPFLTPPFVSPNGFQESPPGVLSLRVSEPLDTSHGMLALKMGSGL
  TLDKAGNLTSQNVTTVTQPLKKTKSNISLDTSAPLTITSGALTVATTAPLIVTSGALSVQSQAPLTVQDS
  KLSIATKGPITVSDGKLALQTSAPLSGSDSDTLTVTASPPLTTATGSLGINMEDPIYVNNGKIGIKISGP
  LQVAQNSDTLTVVTGPGVTVEQNSLRTKVAGAIGYDSSNNMEIKTGGGMRINNNLLILDVDYPFDAQTKL
  RLKLGQGPLYINASHNLDINYNRGLYLFNASNNTKKLEVSIKKSSGLNFDNTAIAINAGKGLEFDTNTSE
  SPDINPIKTKIGSGIDYNENGAMITKLGAGLSFDNSGAITIGNKNDDKLTLWTTPDPSPNCRIHSDNDCK
  FTLVLTKCGSQVLATVAALAVSGDLSSMTGTVASVSIFLRFDQNGVLMENSSLKKHYWNFRNGNSTNANP
  YTNAVGFMPNLLAYPKTQSQTAKNNIVSQVYLHGDKTKPMILTITLNGTSESTETSEVSTYSMSFTWSWE
  SGKYTTETFATNSYTFSYIAQE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000007
• ORF Size: 1746 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000007.1, AP_000190
• RefSeq ORF: 1746
• MW: 61.9 kDa