E4 ORF1 (AC_000005) Virus Tagged ORF Clone

CAT#: VC100253

Myc-DDK-tagged ORF clone of viral ORF for E4 ORF1 [Human adenovirus A], codon optimized for human cell expression, AP_000141

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4 ORF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100253 represents NCBI reference of AP_000141 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCCTTCGAAACACTTTACGTGTATTTTACCGGGCCGGGCGCTATGCTGCCCAAGCAGGAAGGCG
  ACAGCAATGCCTATGTGCTGTTTTCTCCCGCAAACTTCGTTATTCCGCCTCATGGCGTAGTTCTTCTGTA
  TCTTCATATAGCCGTGGATATTCCACCCGGTTACTTGGGTACCCTTTTTTCTCTGTGCGATATGAATGCT
  CGGGGCGTCTTTGTTGGCGCAGAGACCCTCTACCCGGGGTCAAGGATGGAACTCTCAGTGCTCCTGTTTA
  ATCACTCCGACGTGTTCTGCGACGTGAGAGCAAAGCAGCCGGTGGCCAGACTCCTCCTTAGCCGCGTTGT
  GTTCCCCCCCGTGTGCCAAGCCAGCCTGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100253 representing AP_000141
  Red=Cloning sites Green=Tags
  
  MAAFETLYVYFTGPGAMLPKQEGDSNAYVLFSPANFVIPPHGVVLLYLHIAVDIPPGYLGTLFSLCDMNA
  RGVFVGAETLYPGSRMELSVLLFNHSDVFCDVRAKQPVARLLLSRVVFPPVCQASLI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000005
• ORF Size: 381 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000005.1, AP_000141
• RefSeq ORF: 381
• MW: 13.9 kDa