DBP (AC_000005) Virus Tagged ORF Clone

CAT#: VC100235

Myc-DDK-tagged ORF clone of viral ORF for DBP [Human adenovirus A], codon optimized for human cell expression, AP_000123

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DBP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100235 represents NCBI reference of AP_000123 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCTAACCAGCACAGTCAGCGAGAACGGACGCCCGACAGATCTGCGCAGCCACCCCCCCCCAAGA
  TGGGCAGATACTTCCTTGACAGCGAATCCGAGGAGGAGTTGGAGGCCGTCCCCCTCCCTCCAAAGAAGAA
  GGTCAAGAAGTCAATGGCAGCCATTCCACTTTCACCTGAGTCCGCAGAGGAGGAGGAAGCCGAGCCCCCC
  AGAGCTGTCCTGGGCGTCATGGGCTTCTCCATGCCTCCGGTCAGGATCATGCATCATGCTGATGGGAGTC
  AATCATTTCAGAAGATGGAGACGAGACAGGTGCATGTATTGAAAGCTTCCGCCCAGAACTCAGACGAAAA
  CGAAAAGAACGTGGTTGTGGTGCGGAACCCAGCCAGCCAGCCCCTGGTGAGCGCCTGGGAGAAGGGAATG
  GAAGCTATGGCTATGCTGATGGAGAAATATCACGTGGACCACGATGAAAGGGCCACCTTTAGATTTCTTC
  CAGATCAGGGATCCGTGTATAAGAAAATCTGCACCACATGGCTCAACGAAGAGAAGAGGGGGCTGCAACT
  CACCTTCTCATCCCAGAAAACTTTTCAAGAGCTCATGGGGCGGTTTCTGCAGGGTTACATGCAGGCCTAT
  GCAGGGGTACAACAGAATTCATGGGAGCCGACCGGCTGTTGCGTCTGGGAGCATAAGTGCACGGAGAGAG
  AAGGCGAACTGCGATGTCTCCACGGAATGGAGATGGTGAGAAAAGAACATTTGGTAGAAATGGATGTCAC
  AAGCGAGTCAGGCCAAAGAGCGCTCAAGGAGAATCCCTCCAAAGCCAAAGTGGCCCAGAATAGATGGGGC
  AGGAACGTGGTTCAGATCAAGAACGACGACGCAAGGTGCTGTTTCCACGACGTCGGATGCGGAAATAATT
  CCTTCAGTGGCAAGTCTTGTGGGCTCTTCTACTCAGAAGGAATGAAGGCCCAGATCGCATTCAGACAGAT
  CGAAGCATTTATGCTCGCTGATTATCCCCATATGCGGCACGGCCAGAAGCGGCTGCTCATGCCGGTGCGA
  TGCGAATGCTTGAATAAGCAAGACGGGTTGCCAAGGATGGGACGGCAGCTGTGCAAAATTACTCCCTTTA
  ACTTGTCAAACGTGGATAATATCGATATCAACGAGGTCACCGATCCAGGTGCTCTTGCCTCAATTAAATA
  CCCATGTCTGCTGGTCTTTCAGTGCGCAAACCCAGTTTACCGGAATGCTAGAGGCAATGCTGGCCCAAAC
  TGTGATTTCAAGATCTCAGCACCAGATGTAATGGGCGCACTTCAGCTGGTCCGCCAGCTGTGGGGAGAGA
  ATTTCGACGGGTCCCCTCCACGGCTTGTCATTCCGGAGTTTAAGTGGCACCAGAGACTCCAGTACAGAAA
  TATCAGCCTCCCAACAAATCATGGTGACTGCAGGGAAGAACCGTTCGACTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100235 representing AP_000123
  Red=Cloning sites Green=Tags
  
  MASNQHSQRERTPDRSAQPPPPKMGRYFLDSESEEELEAVPLPPKKKVKKSMAAIPLSPESAEEEEAEPP
  RAVLGVMGFSMPPVRIMHHADGSQSFQKMETRQVHVLKASAQNSDENEKNVVVVRNPASQPLVSAWEKGM
  EAMAMLMEKYHVDHDERATFRFLPDQGSVYKKICTTWLNEEKRGLQLTFSSQKTFQELMGRFLQGYMQAY
  AGVQQNSWEPTGCCVWEHKCTEREGELRCLHGMEMVRKEHLVEMDVTSESGQRALKENPSKAKVAQNRWG
  RNVVQIKNDDARCCFHDVGCGNNSFSGKSCGLFYSEGMKAQIAFRQIEAFMLADYPHMRHGQKRLLMPVR
  CECLNKQDGLPRMGRQLCKITPFNLSNVDNIDINEVTDPGALASIKYPCLLVFQCANPVYRNARGNAGPN
  CDFKISAPDVMGALQLVRQLWGENFDGSPPRLVIPEFKWHQRLQYRNISLPTNHGDCREEPFDF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000005
• ORF Size: 1452 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000005.1, AP_000123
• RefSeq ORF: 1452
• MW: 55.0 kDa