E1A (AC_000005) Virus Tagged ORF Clone

CAT#: VC100219

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for E1A [Human adenovirus A], codon optimized for human cell expression, AP_000107


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Reconstitution Protocol

USD 400.00

3 Weeks*

Size
    • 10 ug

Product Images

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Symbol E1A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100219 represents NCBI reference of AP_000107 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGAACCGAAATGACTCCCCTCGTTCTGTCCTACCAGGAGGCTGACGACATCCTGGAGCACTTGGTGG
ACAACTTCTTTAACGAGGTGCCTTCCGATGACGATCTGTACGTTCCCTCTCTCTATGAACTGTATGATCT
CGATGTGGAAAGCGCCGGCGAGGATAACAACGAACAGGCAGTAAATGAGTTTTTCCCAGAGTCCCTTATC
TTGGCCGCGTCCGAAGGACTGTTTCTGCCCGAGCCCCCCGTCTTGAGCCCTGTGTGCGAACCCATTGGCG
GAGAATGTATGCCCCAGCTGCACCCTGAGGATATGGATCTCTTGTGCTATGAAATGGGTTTTCCATGCTC
TGACAGCGAGGACGAACAGGATGAAAACGGAATGGCACATGTGTCCGCCAGTGCTGCAGCAGCTGCTGCT
GATCGGGAACGGGAGGAGTTCCAGCTGGACCACCCAGAGCTTCCAGGCCATAACTGCAAGAGCTGCGAGC
ACCACCGCAATTCCACCGGTAACACAGATCTGATGTGTTCCTTGTGTTATCTTAGAGCCTATAACATGTT
CATTTATAGCCCCGTTTCAGACAACGAGCCAGAGCCCAATAGCACCCTCGACGGAGATGAAAGGCCAAGC
CCTCCTAAGTTGGGATCCGCTGTACCCGAGGGAGTCATCAAACCCGTTCCTCAGCGCGTGACCGGTCGCA
GAAGGTGCGCCGTGGAGTCAATCTTGGATTTGATTCAGGAAGAGGAAAGAGAACAGACAGTGCCGGTTGA
CCTTTCCGTGAAAAGACCTCGATGCAAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100219 representing AP_000107
Red=Cloning sites Green=Tags

MRTEMTPLVLSYQEADDILEHLVDNFFNEVPSDDDLYVPSLYELYDLDVESAGEDNNEQAVNEFFPESLI
LAASEGLFLPEPPVLSPVCEPIGGECMPQLHPEDMDLLCYEMGFPCSDSEDEQDENGMAHVSASAAAAAA
DREREEFQLDHPELPGHNCKSCEHHRNSTGNTDLMCSLCYLRAYNMFIYSPVSDNEPEPNSTLDGDERPS
PPKLGSAVPEGVIKPVPQRVTGRRRCAVESILDLIQEEEREQTVPVDLSVKRPRCN

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN AC_000005
ORF Size 798 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Reference Data
RefSeq AC_000005.1, AP_000107
RefSeq ORF 798
MW 29.7 kDa

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.