E1A (AC_000005) Virus Tagged ORF Clone

CAT#: VC100219

Myc-DDK-tagged ORF clone of viral ORF for E1A [Human adenovirus A], codon optimized for human cell expression, AP_000107

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100219 represents NCBI reference of AP_000107 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAACCGAAATGACTCCCCTCGTTCTGTCCTACCAGGAGGCTGACGACATCCTGGAGCACTTGGTGG
  ACAACTTCTTTAACGAGGTGCCTTCCGATGACGATCTGTACGTTCCCTCTCTCTATGAACTGTATGATCT
  CGATGTGGAAAGCGCCGGCGAGGATAACAACGAACAGGCAGTAAATGAGTTTTTCCCAGAGTCCCTTATC
  TTGGCCGCGTCCGAAGGACTGTTTCTGCCCGAGCCCCCCGTCTTGAGCCCTGTGTGCGAACCCATTGGCG
  GAGAATGTATGCCCCAGCTGCACCCTGAGGATATGGATCTCTTGTGCTATGAAATGGGTTTTCCATGCTC
  TGACAGCGAGGACGAACAGGATGAAAACGGAATGGCACATGTGTCCGCCAGTGCTGCAGCAGCTGCTGCT
  GATCGGGAACGGGAGGAGTTCCAGCTGGACCACCCAGAGCTTCCAGGCCATAACTGCAAGAGCTGCGAGC
  ACCACCGCAATTCCACCGGTAACACAGATCTGATGTGTTCCTTGTGTTATCTTAGAGCCTATAACATGTT
  CATTTATAGCCCCGTTTCAGACAACGAGCCAGAGCCCAATAGCACCCTCGACGGAGATGAAAGGCCAAGC
  CCTCCTAAGTTGGGATCCGCTGTACCCGAGGGAGTCATCAAACCCGTTCCTCAGCGCGTGACCGGTCGCA
  GAAGGTGCGCCGTGGAGTCAATCTTGGATTTGATTCAGGAAGAGGAAAGAGAACAGACAGTGCCGGTTGA
  CCTTTCCGTGAAAAGACCTCGATGCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100219 representing AP_000107
  Red=Cloning sites Green=Tags
  
  MRTEMTPLVLSYQEADDILEHLVDNFFNEVPSDDDLYVPSLYELYDLDVESAGEDNNEQAVNEFFPESLI
  LAASEGLFLPEPPVLSPVCEPIGGECMPQLHPEDMDLLCYEMGFPCSDSEDEQDENGMAHVSASAAAAAA
  DREREEFQLDHPELPGHNCKSCEHHRNSTGNTDLMCSLCYLRAYNMFIYSPVSDNEPEPNSTLDGDERPS
  PPKLGSAVPEGVIKPVPQRVTGRRRCAVESILDLIQEEEREQTVPVDLSVKRPRCN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000005
• ORF Size: 798 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000005.1, AP_000107
• RefSeq ORF: 798
• MW: 29.7 kDa