V (AC_000005) Virus Tagged ORF Clone

CAT#: VC100230

Myc-DDK-tagged ORF clone of viral ORF for V [Human adenovirus A], codon optimized for human cell expression, AP_000118

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: V
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100230 represents NCBI reference of AP_000118 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCAAAAGGAAGTTCAAGGAAGAGCTCCTGCAGGCACTGGCCCCAGAAATTTATGGCCCGTCCGACA
  ATCTGACAAAAAGAGACATTAAGCATGTGAAAAAGAGAGAGAAGAAAGAAGAAGAAGTAGCTGCCGCCAG
  CGCCGATGGGGTGGAATTTGTAAGATCTTTTGCCCCCCGACGCAGGGTGCAGTGGAAGGGGAGGCAGGTC
  AAGAGAATACTGAGGCCGGGCACTACTGTCGTTTTCAGTCCAGGTGAGAGAACGATCATGCGCCCCTTGA
  AGAGAGAGTACGATGAAGTCTACGCAGACGATGATATTCTGGAACAGGCAGCTCAGCAGACCGGCGAATT
  CGCATACGGGAAGAAGGGCCGCTACGGGGACAAGATAGCAATTCCACTGGATGAAGGGAATCCGACCCCG
  AGTCTGAAGGCCGTTACTCTCCAGCAGGTGCTCCCCGTGCTGGGACCTAGCGAAGAGAAGCGAGGAATCA
  AACGAGAAGCGATGGACGAGCTGCAGCCAACGATGCAGCTGATGGTTCCTAAGCGACAGAAACTCGAGGA
  TGTACTTGAGCACATGAAGGTGGATCCTTCCGTGCAGCCCGATGTGAAGGTGCGGCCCATAAAAAAGGTA
  GCTCCTGGGCTCGGCGTCCAGACCGTGGATATCCAGATCCCCGTCCAAACGGCACTGGGAGAGACCATGG
  AAATACAGACATCCCCAATCAAAACGACAGTGAATGCGTCAGTGCAGACAGATCCATGGTATCCTCCGGT
  GCTCAGCACGAAGAAGAAGAGGCATTATAGGCAGACATCCTCTCTGCTCCCAGATTATGTGCTCCATCCC
  AGTATCGTCCCTACTCCTGGGTACAGAGGCACCACTTTCCAACGGCGCGCTACGGCCCCATCTCGGCGGA
  GGGGTCCATCAAGGAGAAGAAGGAGAAGGAAGGCGACCCTTGCTCCTGCCGCCGTGCGACGGGTGGTCCA
  GCGCGGGAGGACACTCATCCTGCCTAGTGTGCGGTATCACCCATCTATCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100230 representing AP_000118
  Red=Cloning sites Green=Tags
  
  MTKRKFKEELLQALAPEIYGPSDNLTKRDIKHVKKREKKEEEVAAASADGVEFVRSFAPRRRVQWKGRQV
  KRILRPGTTVVFSPGERTIMRPLKREYDEVYADDDILEQAAQQTGEFAYGKKGRYGDKIAIPLDEGNPTP
  SLKAVTLQQVLPVLGPSEEKRGIKREAMDELQPTMQLMVPKRQKLEDVLEHMKVDPSVQPDVKVRPIKKV
  APGLGVQTVDIQIPVQTALGETMEIQTSPIKTTVNASVQTDPWYPPVLSTKKKRHYRQTSSLLPDYVLHP
  SIVPTPGYRGTTFQRRATAPSRRRGPSRRRRRRKATLAPAAVRRVVQRGRTLILPSVRYHPSIL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000005
• ORF Size: 1032 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000005.1, AP_000118
• RefSeq ORF: 1032
• MW: 39.1 kDa