E3 CR1-beta1 (AC_000005) Virus Tagged ORF Clone

CAT#: VC100241

Myc-DDK-tagged ORF clone of viral ORF for E3 CR1-beta1 [Human adenovirus A], codon optimized for human cell expression, AP_000130

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3 CR1-beta1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100241 represents NCBI reference of AP_000130 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCTCCATCTTTCTGCTGTTTCTCTTCTCACTTCCAAGCGGACTGTACGCGCAGACTGCTGAACGGC
  CTCTCAAAGTTGTGGTGGAGGCGGGTCACAATGTCACACTCCCGCATCTCTCCGGGTCCCATCAAACCGG
  GCATGTCACGTGGCTGGTTGAGACGTCCGACTACGGCAGTGCATCACCCGACAATTTCATCTTTTCTGGC
  CAAAAGCTGTGTCAGTTCACGGATAGAACTATGGTGTGGCCCTATTACAACCTGCACTTTAACTGCGAGA
  ATTACGACCTCAACCTGTTCTGGCTGAAAGTTGAAAATAGTGCCATTTATAACGTGAAGAATACTGTTAA
  CGCCTCTGAAACTAACATCTACTATGACTTGAGGGTGGTTCAGATCTTTCCACCAAAGTGCATAATTACA
  AGCAAATACCTCACTAATGATTACTGCCATATCACCATTAACTGCACGAATAGCGACTACCCTAATAAAG
  TTGTCTTCAATAACGTAAGCAGGTGGTATTATGGTTATGGCAAAGGTAGTCCAACCCTGCCTAATTATTT
  TATTACCAACTTTAACGTTAGCGGGATTACCAAGAGCTTCAACCATACCTATCCGTTTAATGAGCTCTGC
  GACTATCCAACTTCCCAGTCCCAGCACTCTCTGACCCATACCGTCAGCACGGTGATCTTTCTCGGCATCA
  TAGGTTTCTCCATTCTTATTATAATCGCCGCTTTTATCTATCTCTGCTGGCATCGCAAGTCTCTCTGTGT
  CAGCAAAACAGAGCCACTGATGCCTATCCCATAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100241 representing AP_000130
  Red=Cloning sites Green=Tags
  
  MLSIFLLFLFSLPSGLYAQTAERPLKVVVEAGHNVTLPHLSGSHQTGHVTWLVETSDYGSASPDNFIFSG
  QKLCQFTDRTMVWPYYNLHFNCENYDLNLFWLKVENSAIYNVKNTVNASETNIYYDLRVVQIFPPKCIIT
  SKYLTNDYCHITINCTNSDYPNKVVFNNVSRWYYGYGKGSPTLPNYFITNFNVSGITKSFNHTYPFNELC
  DYPTSQSQHSLTHTVSTVIFLGIIGFSILIIIAAFIYLCWHRKSLCVSKTEPLMPIPY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000005
• ORF Size: 804 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000005.1, AP_000130
• RefSeq ORF: 804
• MW: 30.7 kDa