pTP (AC_000005) Virus Tagged ORF Clone

CAT#: VC100225

Myc-DDK-tagged ORF clone of viral ORF for pTP [Human adenovirus A], codon optimized for human cell expression, AP_000113

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pTP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100225 represents NCBI reference of AP_000113 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACTGTCCATTCAGGATTGTGCGCGCCTGACTGGCCAGTCCGTTGCAACCATCGAGAGATTTCTCC
  CCTTGCGCAATATCTGGAATCGAGCCCGGGAATTTATGCGCGCCACAACTACGGCCGCCGGAATCACCTG
  GATGTCTAGGTATATTTACGAATATCATCGCCTCATGCTTGAAGATCTGGCCCCTGGTGCTGCCGCCACT
  CTTGGGTGGCCGCTCTATAGGGAACCACCCCCACACTTCCTGGTAGGCTATCAGTACTTGGTGCGGACCT
  GTAACGATTATGTATTCGATTCAAGAGCTTACTCCCGCCTTCGATATACGGAGACCGTACAGCCCGGAAC
  GCAGACCGTGAACTGGTCCGTGATGACGAATTGCAGTTACACCATCAATGCCGGAGCTTATCATAGATTT
  GTGGACGTCGATGATTTTGCTACGACCCTTACCCAGATTCAACAGGCCGTGTTGGCCGAGCGGGTCGTCG
  CCGACCTTGCCCTGCTGCAGCCCCTGCAGGGGTACGGTAGCACGCACATGGCTGACCGGGGCAATCGCGA
  AGTCGAGGTCGAGCGACTCATGCAGGACTATTATAAGGACCTGGGACGGTGCCAGAGCGACGTCTGGGGT
  ATGGCAGATCGCTTGCGAATTCAGCAAGCTGGACCTAAGGATGTGGTGCTGCTGAAAACAATAAGGGGCC
  TGAAGACCGCCTACTTCAATTACATCATCTCATCCATTGCACAGCTTCCCGGAACGCTGGAGGAGACAAA
  ATTGTCTTTGCCATGTGATTGTGACTGGCTCGATGCATTCCTGGAGCGGTTCAGCGACCCAGTCGACATG
  CAAACACTGTCTAGTCTGAGGGGAGTTCCCACACAACAAATCATTAAGTGTATAATTTCAGCCGTCAGTC
  TGCCCAACGCTCCTCGAAACGCTTCATTCCCAATCCTTCACGAGACAGAGTTGCGCGGCGGAGTGTTTGA
  GCTTAGACCAAGGGAGAACGGTCGGGCTGTGACCGAAACTATGCGAAGACGACGCGGGGAGATGATTGAG
  CGATTTGTGGATCGCCTGCCGGTGCGCCGACGACGCAGACGCCAGCCTCCCCCACCAGTGGTGGAGGAGG
  AAATCATGGAAGTGGAGGAGGAGGAGCAAGAGATCGTGCCCGGTGCTTTCCAGCAGGAGGTGCGAGAAAC
  AGTGGCTGAACTGATTAGACTGCTTGAGGAGGAGTTGACGGTGGCCGCGAGGAATTCACAATTCTTCAAT
  TTTGCGGTAGATTTTTATGAGGCCATGGAGCGCTTGGAGGCATTGGGAGACATCAACGAACTCACACTCA
  GACGGTGGATCCTCTATTTCTTCGTCTCCGAGCACGTGGCCACCACTCTGAACTACCTGTTTCAGAGGCT
  TCGCAACTACGGCGTGTTTGCCAGACTGGTGGAGCTGAACCTCGCGCAAGTTGTTATGAGAGCTCGAGAT
  TCAGAAGGCGGAGTCGTGTATAGCCGAGTGTGGAACGAAATCGGGCTCAACGCTTTCTCCCAGCTGATGA
  GTAGGATTTCAAATGACCTCGCTGCTACTATTGAAAGGGCGGGCCATGGAGACTTGCAGGAGGAGGAGAT
  CGACCAGCTGATGGCAGAAATCGCTTACCAGGATAACTCCGGCGACGTACAGGAAATTTTGAGACAGGCA
  GCCGTGAACGACACTGAAATTGATTCCGTGGAGCTGTCATTCCGCTTCAAAACTACAGGCCCGGTTGTGT
  TTACTCAGAGGAGACAGATACAGGACATTAACCGGCGCGTGGTAGCTCACGCCAGCCAGCTTAGAGCACA
  GCATCTGCCCCTGCCAGAGAGACAAGCTGACATTCCTCTCCCCCCACTGCCCGCCGGACCAGAGCCACCT
  CTCCCCCCGGGAGCCAGACCTCGGAGAAGGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100225 representing AP_000113
  Red=Cloning sites Green=Tags
  
  MALSIQDCARLTGQSVATIERFLPLRNIWNRAREFMRATTTAAGITWMSRYIYEYHRLMLEDLAPGAAAT
  LGWPLYREPPPHFLVGYQYLVRTCNDYVFDSRAYSRLRYTETVQPGTQTVNWSVMTNCSYTINAGAYHRF
  VDVDDFATTLTQIQQAVLAERVVADLALLQPLQGYGSTHMADRGNREVEVERLMQDYYKDLGRCQSDVWG
  MADRLRIQQAGPKDVVLLKTIRGLKTAYFNYIISSIAQLPGTLEETKLSLPCDCDWLDAFLERFSDPVDM
  QTLSSLRGVPTQQIIKCIISAVSLPNAPRNASFPILHETELRGGVFELRPRENGRAVTETMRRRRGEMIE
  RFVDRLPVRRRRRRQPPPPVVEEEIMEVEEEEQEIVPGAFQQEVRETVAELIRLLEEELTVAARNSQFFN
  FAVDFYEAMERLEALGDINELTLRRWILYFFVSEHVATTLNYLFQRLRNYGVFARLVELNLAQVVMRARD
  SEGGVVYSRVWNEIGLNAFSQLMSRISNDLAATIERAGHGDLQEEEIDQLMAEIAYQDNSGDVQEILRQA
  AVNDTEIDSVELSFRFKTTGPVVFTQRRQIQDINRRVVAHASQLRAQHLPLPERQADIPLPPLPAGPEPP
  LPPGARPRRRF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000005
• ORF Size: 1923 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000005.1, AP_000113
• RefSeq ORF: 1923
• MW: 73.5 kDa