III (AC_000005) Virus Tagged ORF Clone

CAT#: VC100227

Myc-DDK-tagged ORF clone of viral ORF for III [Human adenovirus A], codon optimized for human cell expression, AP_000116

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: III
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100227 represents NCBI reference of AP_000116 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGACGGGCAGTCGAACTTCAAACGGTGGCCTTTCCGGAAACCCCTCCCCCCTCTTATGAAACAGTTA
  TGGCCGCGGCGCCTCCTTACGTGCCTCCCAGGTACCTGGGACCGACGGAAGGAAGGAATAGCATACGCTA
  TTCAGAACTGAGCCCTTTGTATGACACAACTCGGGTATACCTGGTGGACAATAAAAGTTCTGACATCGCT
  TCACTGAACTACCAGAATGATCACTCAAACTTCTTGACCACTGTTGTACAGAACAACGACTACTCACCCA
  TTGAGGCTGGCACGCAAACCATCAACTTCGATGAGCGATCCAGGTGGGGCGGGGATTTGAAAACCATCCT
  CCACACGAACATGCCTAATGTCAATGATTTCATGTTTACCACTAAATTCAAGGCCCGCGTCATGGTGGCA
  CGGAAGACCAATAACGAGGGTCAGACCATCCTGGAGTACGAGTGGGCGGAGTTTGTGCTGCCTGAGGGGA
  ATTATTCCGAGACTATGACCATCGACCTGATGAACAACGCAATCATAGAGCACTACCTGCGGGTGGGCCG
  CCAACACGGCGTTCTTGAGTCTGATATAGGCGTCAAATTTGACACTAGAAATTTCCGGCTGGGATGGGAC
  CCTGAAACACAGCTCGTGACTCCAGGCGTATACACCAACGAGGCCTTTCATCCTGACATTGTCCTTCTTC
  CTGGTTGTGGGGTGGACTTCACTGAGTCTAGGCTTAGCAACATTCTGGGGATACGCAAGAGACAGCCATT
  TCAGGAAGGGTTTGTGATAATGTACGAGCATCTGGAAGGCGGGAACATCCCTGCGCTTTTGGATGTCAAG
  AAGTATGAAAATTCTCTCCAGGATCAGAACACAGTGAGAGGGGACAATTTCATAGCCCTTAACAAAGCCG
  CTCGGATTGAACCCGTGGAAACCGATCCTAAGGGGAGATCCTATAACTTGCTCCCCGACAAGAAAAATAC
  TAAGTATCGCAGCTGGTACCTTGCTTATAATTACGGGGATCCTGAGAAGGGCGTGAGAAGCTGGACACTC
  CTCACCACACCCGACGTTACGGGGGGATCAGAGCAGGTTTACTGGTCATTGCCTGATATGATGCAAGATC
  CAGTGACCTTCAGGTCCAGTCGCCAGGTTTCCAATTATCCTGTCGTGGCTGCCGAATTGCTGCCCGTGCA
  TGCCAAGTCCTTCTACAACGAGCAGGCTGTTTATTCTCAGCTCATCCGGCAGTCCACTGCACTCACCAGA
  GTATTCAACCGCTTTCCCGAAAATCAGATACTCGTTAGACCCCCCGCCGCTACAATAACTACTGTCTCCG
  AGAACGTGCCCGCTTTGACCGACCATGGGACATTGCCCTTGAGGTCCAGTATCAGTGGGGTTCAGCGAGT
  CACAATCACAGATGCCAGACGCCGCACCTGCCCTTATGTCTACAAAGCACTGGGCATTGTTTCCCCGCGC
  GTGCTGAGCTCTAGGACCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100227 representing AP_000116
  Red=Cloning sites Green=Tags
  
  MRRAVELQTVAFPETPPPSYETVMAAAPPYVPPRYLGPTEGRNSIRYSELSPLYDTTRVYLVDNKSSDIA
  SLNYQNDHSNFLTTVVQNNDYSPIEAGTQTINFDERSRWGGDLKTILHTNMPNVNDFMFTTKFKARVMVA
  RKTNNEGQTILEYEWAEFVLPEGNYSETMTIDLMNNAIIEHYLRVGRQHGVLESDIGVKFDTRNFRLGWD
  PETQLVTPGVYTNEAFHPDIVLLPGCGVDFTESRLSNILGIRKRQPFQEGFVIMYEHLEGGNIPALLDVK
  KYENSLQDQNTVRGDNFIALNKAARIEPVETDPKGRSYNLLPDKKNTKYRSWYLAYNYGDPEKGVRSWTL
  LTTPDVTGGSEQVYWSLPDMMQDPVTFRSSRQVSNYPVVAAELLPVHAKSFYNEQAVYSQLIRQSTALTR
  VFNRFPENQILVRPPAATITTVSENVPALTDHGTLPLRSSISGVQRVTITDARRRTCPYVYKALGIVSPR
  VLSSRTF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000005
• ORF Size: 1491 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000005.1, AP_000116
• RefSeq ORF: 1491
• MW: 56.4 kDa