pol (AC_000005) Virus Tagged ORF Clone

CAT#: VC100224

Myc-DDK-tagged ORF clone of viral ORF for pol [Human adenovirus A], codon optimized for human cell expression, AP_000112

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pol
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100224 represents NCBI reference of AP_000112 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATTGGTTCAAAATAACCGCGCCGGGTCTGTCTACACTACACCAACTGACTCAAGATATCAGTCTC
  CAGGGAGTCGGCCTTGTCAGTCCACTAGCGGTTCTACACCCTCACCAGCCCGAGCCTCCGGTCGGCATAC
  CGCCTCACCCGCTAGTCGGAGGACGAGAACTGCCGCATCTAGTGGCTCTTCTTCAGCACCACCAATCCTG
  AGCCGGCGAATCGGGAAGGTGCACCGGGGGACCGTGGTGTCACCCAGAGCGCATGGAATCCTGCACGCCA
  TCGACAGCGCCAACGGTACACCCCTGGAGATCAAGTACTACTTGCACCTCCAACCAGCATTGACGCGCCT
  GTGTGAGGTGAATCTGAGGGCCGTGCCAGCTGATATGTTGTTTTCACTCACTGATACTATGGACAGCAGC
  AGGCTGTACGCTCTGATCTCCCGATTCAGGCCCAGTAGAGCTGAGATCTGGACATGTCGCAGCAGGGGGA
  CGGTTACCCTCAGCGTCCTCGCCTTCGAGGACCCCAATGGAAGCGGGAATGCCGCCGAGGAGGAAGAGGA
  CGAGCGACAGCTGCCTAGCGGGATCGACTTTCCTATTTGCTTCCTGGTACGGGGAAGACAAGTGCACCTT
  ATCCAGCAAATTCAGCCTGTGCAGCGGTGCGAGCACTGCGCACGGTTCTATAAGCATCAGCACGAGTGCT
  CAGTCAGAAGACGGGACTTCTACTTCCATCATATCAATTCTCACAGCAGCAACTGGTGGCAGGAAATTCA
  CTTCTTTCCAATCGGAAGCCACCCCAGAACGGAGCGCCTGTTCATTACCTACGACGTCGAGACATATACG
  TGGATGGGCGCCTTTGGTAAACAGCTCGTCCCATTCATGCTGGTTATGAAGCTCTCCGGAGACGATAACT
  TGGTTAAACACGCTCTCCAGCTGGCGCTCGAGTTGGGATGGGACCAGTGGGAAAAGGATTCCACCACATT
  TTACTGCCTGACCCCGGAAAAGATGAAGGTCGGCCAACAGTTCCGCACATACCGCAATCGGCTGCAGACT
  TCTCTCGCCACCGATTTGTGGATGACGTTTCTTCAAAAGAATCCGCATCTTTCTCAGTGGGCACAGGAGG
  AGAATGGTCTGGTTGCCCTGGAGGACCTCTCTTACGAAGACTTGAAGCGGGCACCCGCTATTAAGGGCGA
  ACCGCGGTTTGTAGAACTGTATATCGTTGGACATAATATCAATGGGTTCGACGAGATCGTTTTGGCAGCC
  CAGGTCATTAATAACAGACTCGATGTCCCTGGACCCTTCAAAATTAGCCGGAATTTCATCCCTCGGGCCG
  GGAAAATCCTGTTCAACGACATTACATTTGCGCTTCCGAACCCACATTACAAGAAGCGCACAAATTTTCT
  CCTGTGGGAGCACGGCGGCTGCGATGACCAAGATTTCAAGTATCAGTACCTGAAAGTCATGGTGCGCGAC
  ACTTTTGCCCTGACCCACACTTCCCTCAGGAAAGCTGCACAGGCCTACGCTCTGCCCGTGGAAAAAGGAT
  GCTGCCCATACAAAGCTGTGAATCAGTTTTACATGCTGGGTTCATATCGCGCCGACGCTAACGGTTTCCC
  CCTTGAGGAGTATTGGAAGGATAAGGAAGAATACCTGCTGAATCAAGAACTGTGGAAAAAGAAGGGCGAG
  AAGAATTACAATCTCATCGGAGAGACTTTGAATTATTGCGCTCTGGACGTTCTGGTCACCGCAAGCTTGG
  TCGAAAAGCTGCGCTCCTCCTACGCTCAGTTCGTGACGGATGCAGTCGGGCTCGATGCAGCCCATTTTAA
  TGTGTTTCAGCGACCCACTATCAGTAGCAACTCTCATGCCATATTCAGGCAAATTCTGTACAGAGCAGAG
  AAGCCGCAGAGGACCCACCTCGGCCCCAACATCCTGGCCCCCTCCCATGAGCTGTATGACTACGTGAGAG
  CAAGTATACGAGGTGGGCGGTGTTACCCTACATACATTGGTGTGCTGAAAGAACCCATATACGTGTATGA
  CATTTGTGGCATGTACGCGAGCGCCCTGACTCATCCCATGCCTTGGGGACCTCCACTGAATCCGTATGAG
  CGAGCGCTCGCCGTCAGGCAGTGGCAGGTGGCACTTGAGAATTACACTTGCAAGATCGACTATTTTGACA
  AGAATCTGTGCCCTGGCATCTTCACCATCGATGCTGATCCTCCTGATGAAAACCAGCTGGACGTCCTTCC
  GCCATTCTGCTCAAGGAAAGGCGGTAGGCTGGCCTGGACAAATGAAAGTCTGAGGGGAGAGGTTGTGACG
  AGCGTGGACTTGGTTACGCTGCATAATAGGGGGTGGCGCCTCAGACTGCTGTCAGACGAAAGAACCACCA
  TCTTCCCTACTTGGAAGTGTTTGGCCCGGGAGTACGTGCAGCTTAATATCGCAGCCAAGGAACGGGCCGA
  CAGGGACAAGAACCAAACTCTGAGGTCTATCGCAAAACTGCTTAGCAACGCTCTTTATGGATCCTTCGCT
  ACCAAACTTGACAATAAGAAAATAGTATTCAGCGATCAGATGGAGGAGAGCCTTATGAAGGAGATCGCTG
  CAGGCCGGCTGAACATTAAATCTTCCTCTTTCATAGAGACGGATACGCTGAGTACTGAGGTCATGCCCGC
  TTTCGAGAGGGTCTACAGCCCAAACCAGCTGGCCCTGGTGAATTCAGAGGCCGAAGAAAGCGATGAGGAC
  CAAGGTCCCGCCCCCTTCTACAGTCCACCTCCCGAAAACTGTGAACACGTGACTTACACCTATAAACCTA
  TCACTTTTATGGACGCTGAGGAAGGCGATATGTGTCTCCATACTCTGGAGTCATCCAATCCTCTGATTAA
  TAATGACAGATACCCAAGCCATGTGGCATCATTCGTACTCGCTTGGACACGGGCATTTGTATCTGAATGG
  AGTGAATTTCTGTACGAAGAGGACAGGGGCATTCCCCTTAAGGACCGACCGCTGAAATCTGTGTACGGCG
  ACACTGACTCTCTGTTTGTTACTGAGAAGGGGAGGAGATTGATGGAGAGCCAGGGAAAGAAGCGCATTAA
  AAAGTACGGAGGAAAGTTGGTCTTCGACCCTAGTTGTCCAGAATTGACATGGAGCGTCGAATGCGAGACC
  GTGTGTTCTTATTGTGGCGCTGACGCCTACAGCCCGGAGAGCGTATTTCTGGCTCCGAAACTCTATGCGC
  TGAAGTGTCTGCAGTGTCCTCACTGCGGTAGTACGAGCAAGGGCAAGATTCGAGCCAAAGGTCACGCCAC
  AGAAGCACTTAGTTATGATCTTATGCTGAAATGCTATCTGGCAGAGGCGCAAGGCGAGGATACTAGGTTC
  AGCACATCTCGACTCTCACTCAAAAGAACACTCGCTAGTGCCCAACCTGGGGCTCACCCATTCACGGTGA
  CCGAGACCACCCTCACCAGGACCCTTAGACCATGGAAAGACAAGACTCTGGTTCATCTGGATGCACACAG
  ACTGGTGCCATATTCTAATAGCCAGCCTAACCCGCGCAATGAGGAGGTGTGTTGGATCGAGATGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100224 representing AP_000112
  Red=Cloning sites Green=Tags
  
  MALVQNNRAGSVYTTPTDSRYQSPGSRPCQSTSGSTPSPARASGRHTASPASRRTRTAASSGSSSAPPIL
  SRRIGKVHRGTVVSPRAHGILHAIDSANGTPLEIKYYLHLQPALTRLCEVNLRAVPADMLFSLTDTMDSS
  RLYALISRFRPSRAEIWTCRSRGTVTLSVLAFEDPNGSGNAAEEEEDERQLPSGIDFPICFLVRGRQVHL
  IQQIQPVQRCEHCARFYKHQHECSVRRRDFYFHHINSHSSNWWQEIHFFPIGSHPRTERLFITYDVETYT
  WMGAFGKQLVPFMLVMKLSGDDNLVKHALQLALELGWDQWEKDSTTFYCLTPEKMKVGQQFRTYRNRLQT
  SLATDLWMTFLQKNPHLSQWAQEENGLVALEDLSYEDLKRAPAIKGEPRFVELYIVGHNINGFDEIVLAA
  QVINNRLDVPGPFKISRNFIPRAGKILFNDITFALPNPHYKKRTNFLLWEHGGCDDQDFKYQYLKVMVRD
  TFALTHTSLRKAAQAYALPVEKGCCPYKAVNQFYMLGSYRADANGFPLEEYWKDKEEYLLNQELWKKKGE
  KNYNLIGETLNYCALDVLVTASLVEKLRSSYAQFVTDAVGLDAAHFNVFQRPTISSNSHAIFRQILYRAE
  KPQRTHLGPNILAPSHELYDYVRASIRGGRCYPTYIGVLKEPIYVYDICGMYASALTHPMPWGPPLNPYE
  RALAVRQWQVALENYTCKIDYFDKNLCPGIFTIDADPPDENQLDVLPPFCSRKGGRLAWTNESLRGEVVT
  SVDLVTLHNRGWRLRLLSDERTTIFPTWKCLAREYVQLNIAAKERADRDKNQTLRSIAKLLSNALYGSFA
  TKLDNKKIVFSDQMEESLMKEIAAGRLNIKSSSFIETDTLSTEVMPAFERVYSPNQLALVNSEAEESDED
  QGPAPFYSPPPENCEHVTYTYKPITFMDAEEGDMCLHTLESSNPLINNDRYPSHVASFVLAWTRAFVSEW
  SEFLYEEDRGIPLKDRPLKSVYGDTDSLFVTEKGRRLMESQGKKRIKKYGGKLVFDPSCPELTWSVECET
  VCSYCGADAYSPESVFLAPKLYALKCLQCPHCGSTSKGKIRAKGHATEALSYDLMLKCYLAEAQGEDTRF
  STSRLSLKRTLASAQPGAHPFTVTETTLTRTLRPWKDKTLVHLDAHRLVPYSNSQPNPRNEEVCWIEMA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000005
• ORF Size: 3567 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000005.1, AP_000112
• RefSeq ORF: 3567
• MW: 135.2 kDa