E4 34K (AC_000005) Virus Tagged ORF Clone

CAT#: VC100249

Myc-DDK-tagged ORF clone of viral ORF for E4 34K [Human adenovirus A], codon optimized for human cell expression, AP_000137

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4 34K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100249 represents NCBI reference of AP_000137 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAACGGGACAGGAGGTACAGGTACCGACTGGCCCCTTACAACAAGTATCAGCTCCCTCCTTGTGAAG
  AGCAGTCAAAAGCCACTCTGTCTACCTCCGAGAACAGCCTGTGGCCAGAGTGCAACTCACTCACTTTGCA
  TAACGTGTCTGAAGTGAGGGGGATTCCAAGCTGCGTAGGCTTTACTGTACTCCAGGAATGGCCAATTCCA
  TGGGACATGATCCTCACTGACTATGAAATGTTTATTCTGAAAAAGTATATGTCCGTTTGTATGTGCTGTG
  CCACAATCAACGTGGAAGTCACACAGCTGCTGCACGGCCACGAAAGATGGCTTATTCACTGTCATTGTCA
  GCGGCCTGGATCATTGCAATGTATGAGCGCTGGAATGCTCCTCGGACGGTGGTTTAAGATGGCAGTGTAC
  GGCGCACTGATCAACAAGCGCTGCTTTTGGTACCGCGAGGTGGTTAATCACCTTATGCCAAAAGAGGTTA
  TGTACGTTGGATCCACATTCGTGAGAGGCAGGCATCTCATATATTTTAAAATCATGTACGACGGGCACGC
  TTGGCTCGCTCTCGAAAAAGTGAGCTTTGGTTGGAGTGCGTTTAATTACGGAATACTCAACAACATGTTG
  GTCCTTTGTTGTGACTATTGTAAGGACCTTAGTGAAATACGAATGCGCTGTTGCGCTCGGCGCACTAGGC
  TGCTTATGCTGAAGGTGGTGCAGGTTATTGCGGAAAATACCGTCCGCCCGCTGAAACACTCTCGGCACGA
  GAGATACCGACAGCAGCTGCTTAAGGGCCTGATAATGCACCACAGGGCCATCCTGTTTGGAGACTATAAC
  CAGAGAGAAAATCCGTGGGCAGCAGACGGACAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100249 representing AP_000137
  Red=Cloning sites Green=Tags
  
  MQRDRRYRYRLAPYNKYQLPPCEEQSKATLSTSENSLWPECNSLTLHNVSEVRGIPSCVGFTVLQEWPIP
  WDMILTDYEMFILKKYMSVCMCCATINVEVTQLLHGHERWLIHCHCQRPGSLQCMSAGMLLGRWFKMAVY
  GALINKRCFWYREVVNHLMPKEVMYVGSTFVRGRHLIYFKIMYDGHAWLALEKVSFGWSAFNYGILNNML
  VLCCDYCKDLSEIRMRCCARRTRLLMLKVVQVIAENTVRPLKHSRHERYRQQLLKGLIMHHRAILFGDYN
  QRENPWAADGH
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000005
• ORF Size: 873 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000005.1, AP_000137
• RefSeq ORF: 873
• MW: 34.4 kDa