E1B 19K (AC_000005) Virus Tagged ORF Clone

CAT#: VC100220

Myc-DDK-tagged ORF clone of viral ORF for E1B 19K [Human adenovirus A], codon optimized for human cell expression, AP_000108

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1B 19K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100220 represents NCBI reference of AP_000108 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTTGGAGACGGTCCTTCAGTCCTTTCAGAGTGTGCGCCAACTGCTCCAATACACATCAAAAAACA
  CGAGCGGTTTCTGGAGATACCTTTTCGGATCTACCCTGTCCAAGGTTGTCAACCGAGTGAAAGAAGATTA
  CCGCGAAGAATTCGAAAATATTTTGGCAGACTGCCCAGGCCTCCTTGCCAGTCTGGACTTGTGTTACCAT
  CTGGTGTTCCAAGAGAAGGTGGTTAGGTCCTTGGATTTCAGCTCCGTTGGACGGACCGTTGCTTCAATTG
  CATTCCTGGCTACTATTCTGGATAAATGGTCTGAAAAGAGCCATCTCTCTTGGGATTATATGCTGGACTA
  CATGTCCATGCAACTCTGGCGAGCCTGGTTGAAACGGCGCGTTTGCATTTATAGCCTGGCTCGGCCCCTC
  ACTATGCCGCCCCTCCCTACACTGCAGGAGGAAAAGGAAGAGGAACGAAACCCCGCCGTGGTCGAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100220 representing AP_000108
  Red=Cloning sites Green=Tags
  
  MELETVLQSFQSVRQLLQYTSKNTSGFWRYLFGSTLSKVVNRVKEDYREEFENILADCPGLLASLDLCYH
  LVFQEKVVRSLDFSSVGRTVASIAFLATILDKWSEKSHLSWDYMLDYMSMQLWRAWLKRRVCIYSLARPL
  TMPPLPTLQEEKEEERNPAVVEK
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000005
• ORF Size: 489 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000005.1, AP_000108
• RefSeq ORF: 489
• MW: 19.1 kDa