E3 RID-beta (AC_000005) Virus Tagged ORF Clone

CAT#: VC100243

Myc-DDK-tagged ORF clone of viral ORF for E3 RID-beta [Human adenovirus A], codon optimized for human cell expression, AP_000132

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3 RID-beta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100243 represents NCBI reference of AP_000132 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAACGGCTCTCGTTCTGTTTTTCATGCTCATCCCCGTGTGGGCCAGTTCTTGCCAGCTGCATAAGC
  CATGGAATTTCCTGGACTGTTATACTAAGGAGACTAATTATATCGGTTGGGTGTATGGGATTATGTCCGG
  ACTGGTCTTCGTCTCCTCTGTGGTTAGCCTCCAACTCTACGCAAGACTCAACTTCTCATGGAATAAGTAC
  ACGGACGACCTTCCAGAATACCCTAATCCACAGGATGATCTGCCTTTGAACATCGTCTTCCCAGAACCCC
  CCAGGCCTCCCTCAGTGGTGAGCTACTTCAAATTCACCGGAGAGGATGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100243 representing AP_000132
  Red=Cloning sites Green=Tags
  
  MKTALVLFFMLIPVWASSCQLHKPWNFLDCYTKETNYIGWVYGIMSGLVFVSSVVSLQLYARLNFSWNKY
  TDDLPEYPNPQDDLPLNIVFPEPPRPPSVVSYFKFTGEDD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000005
• ORF Size: 330 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000005.1, AP_000132
• RefSeq ORF: 330
• MW: 12.8 kDa