pVII (AC_000005) Virus Tagged ORF Clone

CAT#: VC100229

Myc-DDK-tagged ORF clone of viral ORF for pVII [Human adenovirus A], codon optimized for human cell expression, AP_000117

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pVII
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100229 represents NCBI reference of AP_000117 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTATCCTGGTGAGCCCATCCAATAACACCGGATGGGGACTTGGTGCTGCGCGGATGTATGGCGGCG
  CCAAAACTCGATCTAGTCAACATCCGGTTAGGGTGAGGGGCCATTATAGAGCTCCCTGGGGGGCTCACAC
  CCGGGGCCGGACCGGGCGCACTACTGTGGACGATGTGATTGATAGCGTAGTGGCGGATGCTCGGAAATAC
  AGAGCCCCGGCTGAGACGGCTGGATCTACAGTAGACGCCGTAATCGATGAAGTGGTGGCTAACGCCCGGG
  CATATGCTCGAAGGAGAAGGAGACTCAGACGCCGGCGACGCCCCACAACTGCAATGCGAGCAGCACGGGC
  GCTGGTGAGACGCGCCAGACGGATTGGGCGACGAGCAATGATGCGAGCAGCCCGAAGAGCCGCCACACCC
  GCCGGCAGAGCTAGACGAAGAGCCGCTGCAGCAGCAGCTACTGCTATTGCTAACCTGGCTGCCCCCCGGC
  GGGGCAACGTGTACTGGGTTAGAGACAGTGTCACCGGTACGCGGGTCCCCGTGCGGACCCGCCCTCCACA
  CCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100229 representing AP_000117
  Red=Cloning sites Green=Tags
  
  MSILVSPSNNTGWGLGAARMYGGAKTRSSQHPVRVRGHYRAPWGAHTRGRTGRTTVDDVIDSVVADARKY
  RAPAETAGSTVDAVIDEVVANARAYARRRRRLRRRRRPTTAMRAARALVRRARRIGRRAMMRAARRAATP
  AGRARRRAAAAAATAIANLAAPRRGNVYWVRDSVTGTRVPVRTRPPHP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000005
• ORF Size: 564 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000005.1, AP_000117
• RefSeq ORF: 564
• MW: 20.7 kDa