52K (AC_000005) Virus Tagged ORF Clone

CAT#: VC100226

Myc-DDK-tagged ORF clone of viral ORF for 52K [Human adenovirus A], codon optimized for human cell expression, AP_000114

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 52K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100226 represents NCBI reference of AP_000114 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATCCTGTCCTTCGGCAAATGAGGCCTCAGCCACGAGCCACTACCGCATCTGCGGCCGTGGCATTGA
  GCGGATCTGGCGAACAGGAAGAGCCCCAGTGCCCTACTCTGGAGCTGGAGGAGGGCGAGGGTATCGCCCG
  GCTGGGTGCACATTCACCTGAAAGACATCCCCGCGTGCAGCTGGCCCGAGACTCTCGAGTGGCTTTCGTG
  CCTCGGCAAAACATGTTTCGAGACAATTCTGGTGAGGAAGCAGAAGAAATGCGAGACTGTAGGTTCCGAG
  CCGGCCGGGAACTCCGAAGAGGCTTCAATCGGGAGCGGCTGTTGCGGGAAGAAGATTTTGAGCCAGACGA
  ACACTCAGGGATAAGTTCTGCAAGAGCACACGTCAGCGCTGCTAATCTCGTCACAGCATATGAGCAAACC
  GTCACTGAGGAACGGAATTTTCAAAAGAGCTTCAATAATCACGTCCGGACACTCATCGCCAGAGAGGAGG
  TCGCCATTGGGTTGATGCACCTCTGGGACTTTGTGGAAGCTTATGTGCATAATCCCGCTTCTAAGCCCCT
  CACAGCCCAACTCTTCCTCATTGTTCAGCACTCCAGGGACAACGAGACTTTCAGAGATGCTATGCTCAAC
  ATTGCCGAACCACAGGGAAGATGGTTGCTCGACCTGATTAACATCCTTCAGTCAATTGTCGTACAAGAGA
  GGTCTCTCTCTTTGGCCGATAAGGTGGCGGCCATCAACTACTCCATGCTGTCACTCGGGAAGTTCTACGC
  ACGGAAAATTTACAAATCTCCTTACGTCCCAATCGACAAAGAGGTGAAAATCGATAGCTTCTATATGAGA
  ATGGCCCTGAAGGTGCTGACGCTCAGCGACGATTTGGGCGTATACCGGAATGATAGAATTCACAAGGCGG
  TATCCGCTAGCCGCCGGCGGGAATTGAGCGATAAGGAGCTTATGCACAGTCTTCAGAGAGCCCTGACAGG
  TGCGGGCACAGAGGATGAGTCATTTTTTGATATGGGAGCCGATCTTCGGTGGCAGCCTAGCGCCCGGGCA
  TTGGAGGCTGCAGGAGTGGCGTCTGCCGACGTGACCGGTGATGATGACGACGAAGACCAATACGAAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100226 representing AP_000114
  Red=Cloning sites Green=Tags
  
  MHPVLRQMRPQPRATTASAAVALSGSGEQEEPQCPTLELEEGEGIARLGAHSPERHPRVQLARDSRVAFV
  PRQNMFRDNSGEEAEEMRDCRFRAGRELRRGFNRERLLREEDFEPDEHSGISSARAHVSAANLVTAYEQT
  VTEERNFQKSFNNHVRTLIAREEVAIGLMHLWDFVEAYVHNPASKPLTAQLFLIVQHSRDNETFRDAMLN
  IAEPQGRWLLDLINILQSIVVQERSLSLADKVAAINYSMLSLGKFYARKIYKSPYVPIDKEVKIDSFYMR
  MALKVLTLSDDLGVYRNDRIHKAVSASRRRELSDKELMHSLQRALTGAGTEDESFFDMGADLRWQPSARA
  LEAAGVASADVTGDDDDEDQYED
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000005
• ORF Size: 1119 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000005.1, AP_000114
• RefSeq ORF: 1119
• MW: 42.3 kDa