fiber (AC_000005) Virus Tagged ORF Clone

CAT#: VC100246

Myc-DDK-tagged ORF clone of viral ORF for fiber [Human adenovirus A], codon optimized for human cell expression, AP_000135

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: fiber
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100246 represents NCBI reference of AP_000135 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAGATCCCGCACTCAGTACGCGGAGGAAACAGAAGAAAATGATGACTTTAATCCTGTATATCCTT
  TCGACCCATTCGACACTTCTGATGTACCCTTCGTCACTCCCCCCTTCACCAGCAGCAACGGGTTGCAAGA
  GAAGCCGCCAGGCGTGCTGGCTCTGAACTACAAAGACCCCATTGTAACGGAGAATGGAACTTTGACACTG
  AAATTGGGCGACGGCATTAAACTGAATGCCCAGGGGCAGCTCACCGCTAGTAATAACATCAATGTACTCG
  AACCACTGACGAACACATCACAGGGACTGAAGCTTAGTTGGTCCGCACCCTTGGCAGTTAAAGCATCCGC
  GCTCACACTGAACACTCGCGCCCCACTGACTACAACAGACGAATCTCTCGCCCTGATCACCGCCCCACCC
  ATAACAGTAGAATCTTCCAGATTGGGTCTGGCCACCATTGCCCCACTCTCACTCGACGGCGGTGGTAACT
  TGGGCCTGAACCTCTCCGCTCCACTGGACGTCTCTAACAATAACCTGCATTTGACCACTGAGACCCCTCT
  GGTAGTCAACAGCTCCGGCGCCTTGTCAGTCGCCACAGCTGATCCCATTAGTGTGAGGAATAACGCCCTC
  ACCCTGCCAACCGCCGATCCTCTGATGGTCAGCAGCGACGGGCTGGGGATATCAGTAACCAGCCCCATCA
  CAGTGATTAATGGCAGTCTGGCACTCTCCACTACAGCCCCTTTGAATTCTACAGGTAGCACCCTTTCCCT
  CAGTGTGGCGAACCCACTCACTATTAGCCAGGACACATTGACCGTAAGCACGGGAAACGGACTGCAGGTT
  TCAGGATCCCAATTGGTTACTCGGATCGGAGATGGGCTTACTTTCGACAACGGGGTCATGAAGGTTAATG
  TGGCCGGGGGGATGCGGACTAGTGGCGGTCGGATCATCCTGGACGTGAATTATCCCTTTGACGCTTCTAA
  TAATCTCTCCCTGAGAAGGGGTCTGGGCTTGATCTACAACCAATCAACAAATTGGAATCTGACAACTGAT
  ATTTCCACCGAAAAGGGTCTGATGTTCTCAGGAAATCAAATCGCCCTGAATGCTGGGCAAGGGCTGACAT
  TTAATAACGGCCAGTTGAGAGTTAAACTGGGCGCCGGCCTGATATTTGACAGCAATAACAACATCGCACT
  GGGATCTAGCAGTAACACACCTTATGACCCCCTTACCCTGTGGACTACACCGGACCCACCCCCCAATTGC
  TCTCTGATTCAGGAGCTGGATGCAAAGCTGACTCTCTGCCTTACTAAGAATGGCAGCATAGTGAACGGAA
  TAGTGTCTCTCGTGGGGGTGAAGGGAAATCTGCTCAATATCCAGTCTACCACAACCACAGTTGGCGTGCA
  TTTGGTGTTTGACGAGCAAGGGCGCCTGATTACCTCCACACCCACAGCACTGGTGCCGCAGGCATCTTGG
  GGTTACCGCCAGGGCCAAAGTGTGAGCACAAACACAGTCACTAACGGCCTGGGCTTCATGCCCAACGTCA
  GTGCTTATCCCCGACCCAACGCCTCCGAGGCGAAGTCTCAGATGGTGTCACTCACTTACCTGCAAGGGGA
  TACCTCCAAGCCAATTACTATGAAAGTTGCTTTCAATGGCATCACATCCCTTAACGGCTACAGCCTCACG
  TTCATGTGGAGCGGCCTGTCAAACTATATCAATCAGCCGTTTTCCACACCCTCCTGCTCATTTAGCTATA
  TTACACAGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100246 representing AP_000135
  Red=Cloning sites Green=Tags
  
  MKRSRTQYAEETEENDDFNPVYPFDPFDTSDVPFVTPPFTSSNGLQEKPPGVLALNYKDPIVTENGTLTL
  KLGDGIKLNAQGQLTASNNINVLEPLTNTSQGLKLSWSAPLAVKASALTLNTRAPLTTTDESLALITAPP
  ITVESSRLGLATIAPLSLDGGGNLGLNLSAPLDVSNNNLHLTTETPLVVNSSGALSVATADPISVRNNAL
  TLPTADPLMVSSDGLGISVTSPITVINGSLALSTTAPLNSTGSTLSLSVANPLTISQDTLTVSTGNGLQV
  SGSQLVTRIGDGLTFDNGVMKVNVAGGMRTSGGRIILDVNYPFDASNNLSLRRGLGLIYNQSTNWNLTTD
  ISTEKGLMFSGNQIALNAGQGLTFNNGQLRVKLGAGLIFDSNNNIALGSSSNTPYDPLTLWTTPDPPPNC
  SLIQELDAKLTLCLTKNGSIVNGIVSLVGVKGNLLNIQSTTTTVGVHLVFDEQGRLITSTPTALVPQASW
  GYRQGQSVSTNTVTNGLGFMPNVSAYPRPNASEAKSQMVSLTYLQGDTSKPITMKVAFNGITSLNGYSLT
  FMWSGLSNYINQPFSTPSCSFSYITQE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000005
• ORF Size: 1761 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000005.1, AP_000135
• RefSeq ORF: 1761
• MW: 61.7 kDa