pIIIa (AC_000005) Virus Tagged ORF Clone

CAT#: VC100228

Myc-DDK-tagged ORF clone of viral ORF for pIIIa [Human adenovirus A], codon optimized for human cell expression, AP_000115

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pIIIa
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100228 represents NCBI reference of AP_000115 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCGCCCAGCTATAATTGCCGAACGCGCCCCCAATCTCGATCCAGCGGTGCTCGCCGCGATGCAGT
  CTCAACCCTCTGGTGTCACGGCTAGTGATGACTGGACGGCGGCCATGGACCGCATTATGGCTCTGACCGC
  GCGCAGCCCAGATGCCTTCAGACAGCAGCCGCAGGCGAATCGCTTTTCCGCAATACTGGAAGCCGTGGTT
  CCGTCAAGGACTAATCCCACACATGAGAAGGTGCTCACCATAGTTAACGCACTCCTTGATTCAAAGGCAA
  TTCGCAAGGACGAAGCCGGGCTTATTTATAATGCATTGCTCGAACGAGTGGCCCGATATAATTCCACCAA
  CGTTCAAGCTAACCTGGACCGAATGGGCACCGATGTCAAGGAGGCCCTGGCCCAGCGGGAGCGATTTCAT
  CGCGATGGGAACCTGGGATCACTGGTGGCCTTGAATGCCTTCCTCTCCACCCAGCCTGCCAACGTACCTA
  GAGGTCAGGAGGATTACACCAATTTCATTTCTGCCCTGAGACTCATGGTCACCGAAGTGCCCCAATCTGA
  AGTGTATCAAAGTGGCCCTGACTATTTCTTCCAGACCTCTAGGCAGGGTCTGCAGACCGTGAATTTGACC
  CAAGCATTTAAGAACCTGCAAGGATTGTGGGGTGTGCGAGCTCCCGTTGGCGATAGGAGTACTCTGTCAT
  CCCTGTTGACACCAAATTCACGGCTGCTCCTTCTCCTCATCGCTCCCTTCACAAACACAAATTCTTTGTC
  ACGGGATAGCTACCTGGGGCATCTTGTGACTCTGTACCGGGAAGCAATCGGACAGGCCCAGGTGGACGAA
  CAGACATATCAGGAGATCACATCCGTGAGTAGGGCACTGGGCCAAGAAGACACTGGAAGTCTTGAGGCTA
  CCCTTAATTTCTTGCTGACTAATAGAAGACAGCAAGTGCCTCCCCAATACACCCTCAACGCTGAAGAGGA
  ACGAATTCTCCGATACGTTCAGCAGTCCGTGAGTCTTTATTTGATGAGGGAGGGTGCCACTCCTAGCGCG
  GCACTGGATATGACCGCTAGGAACATGGAACCCTCTTTCTACGCAAGCAACCGGGCCTTCATCAACAGAT
  TGATGGATTATCTGCACCGGGCAGCCGCGATGAACGGAGAATATTTCACCAACGCCATATTGAACCCCCA
  CTGGTTGCCCCCCCCTGGCTTCTACACCGGCGAGTTCGATCTTCCCGAAGGAAATGACGGCTTCCTGTGG
  GACGACGTTACGGACTCTCTGTTTAGCCCTGCCGTGATCGGGCATCACGGCAAGAAGGAAGCCGGGGATG
  AGGGTCCCCTGCTCGACTCTAGGGCCTCTTCTCCTTTCCCCTCACTTACCTCACTGCCCGCCTCCGTCAA
  TAGCGGCAGGACAACAAGACCGAGGCTCACCGGTGAATCCGAGTATCTGAATGACCCCATCCTGTTCCCC
  GTCCGCGATAAAAATTTCCCCAACAATGGCATTGAGTCTCTGGTGGATAAAATGTCCAGATGGAAAACTT
  ATGCACAGGAACGGCGCGAATGGGAAGAGAGGCAACCAAGGCCAGTGAGACCGCCCCGCCAGAGGTGGCA
  GCGACGCAAAAAAGGTGCCCACGCCGGAGACGAGGGTTCCGACGACAGCGCTGACGACTCCTCTGTTTTG
  GATCTCGGCGGGAGTGGAAATCCGTTTGCCCATCTCCGGCCTCAGGGTTGTATCGGCTCACTGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100228 representing AP_000115
  Red=Cloning sites Green=Tags
  
  MQRPAIIAERAPNLDPAVLAAMQSQPSGVTASDDWTAAMDRIMALTARSPDAFRQQPQANRFSAILEAVV
  PSRTNPTHEKVLTIVNALLDSKAIRKDEAGLIYNALLERVARYNSTNVQANLDRMGTDVKEALAQRERFH
  RDGNLGSLVALNAFLSTQPANVPRGQEDYTNFISALRLMVTEVPQSEVYQSGPDYFFQTSRQGLQTVNLT
  QAFKNLQGLWGVRAPVGDRSTLSSLLTPNSRLLLLLIAPFTNTNSLSRDSYLGHLVTLYREAIGQAQVDE
  QTYQEITSVSRALGQEDTGSLEATLNFLLTNRRQQVPPQYTLNAEEERILRYVQQSVSLYLMREGATPSA
  ALDMTARNMEPSFYASNRAFINRLMDYLHRAAAMNGEYFTNAILNPHWLPPPGFYTGEFDLPEGNDGFLW
  DDVTDSLFSPAVIGHHGKKEAGDEGPLLDSRASSPFPSLTSLPASVNSGRTTRPRLTGESEYLNDPILFP
  VRDKNFPNNGIESLVDKMSRWKTYAQERREWEERQPRPVRPPRQRWQRRKKGAHAGDEGSDDSADDSSVL
  DLGGSGNPFAHLRPQGCIGSLY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000005
• ORF Size: 1746 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000005.1, AP_000115
• RefSeq ORF: 1746
• MW: 64.8 kDa