E3 14.7K (AC_000005) Virus Tagged ORF Clone

CAT#: VC100244

Myc-DDK-tagged ORF clone of viral ORF for E3 147K [Human adenovirus A], codon optimized for human cell expression, AP_000133

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3 14.7K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100244 represents NCBI reference of AP_000133 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTGAGCCTGACTTGGAGATTGATGGCAGAATCACTGAGCAGAGATTGTTGACTGACCGCGCGCGCC
  GGAGACAGCAGGATCAGAAGAATAAGGAACTGATCGATCTCCAGACTGTGCACCAGTGTAAAAAGGGCCT
  CTTCTGCCTGGTTAAACAAGCCACACTGCGGTACGAAAGTCTGCCTGGAAAGGAACACCAGCTTTGCTAC
  ACACTTCCAACTCAAAGACAAACTTTCACTGCAATGGTGGGCTCAGTGCCCATTAAGGTGTCTCAACAGG
  CTGGGGAACAAGAGGGCAGTATCCGCTGCCTGTGCGACAATCCCGAGTGTCTCTACACACTGATTAAGAC
  TCTTTGCGGTCTGCGGAACTTGCTCCCAATGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100244 representing AP_000133
  Red=Cloning sites Green=Tags
  
  MIEPDLEIDGRITEQRLLTDRARRRQQDQKNKELIDLQTVHQCKKGLFCLVKQATLRYESLPGKEHQLCY
  TLPTQRQTFTAMVGSVPIKVSQQAGEQEGSIRCLCDNPECLYTLIKTLCGLRNLLPMN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000005
• ORF Size: 384 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000005.1, AP_000133
• RefSeq ORF: 384
• MW: 14.7 kDa