100K (AC_000005) Virus Tagged ORF Clone

CAT#: VC100236

Myc-DDK-tagged ORF clone of viral ORF for 100K [Human adenovirus A], codon optimized for human cell expression, AP_000124

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 100K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100236 represents NCBI reference of AP_000124 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGATCTGGAACCCCAGGAATCTCTGACTGCCCCCACCGCACCCGCAATTGGTGCTACCGCTGTCA
  TGGAAAAAGACAAGTCCCTGCTCATCCCTCAGGACGCCCCTGTGGAACAGAACCTCGGGTACGAAACTCC
  ACCCGAAGAATTCGAAGGGTTCTTGCAGATCCAGAAGCAGCCAAATGAGCAGAATGCCGGCCTTGAGGAT
  CACGATTATCTTAATGAGGGAGACGTGCTTTTCAAGCATCTGCAAAGACAGAGTACCATCGTGCGGGATG
  CCATTAGTGACAGAAGCAGCATACCCGTATCCATTGCCGAACTGTCTTGTATTTACGAGAGGAACCTTTT
  TTCCCCCAGAGTCCCACCCAAACGACAAGCTAACGGAACTTGTGAACCTAACCCCAGGCTGAACTTTTAC
  CCAGTGTTCGCAGTCCCTGAGGCCCTGGCAACCTATCATATCTTTTTCAAAAATCACAAGATTCCATTGT
  CTTGTAGAGCCAACCGAAGCCGGGCGGACGAGCTGCTGGCTCTCAGAGCAGGTGCTTCAATCCCCGGAAT
  AGTCTCCTTGGAGGAGGTGCCCAAAATTTTCGAGGGGCTGGGGAGAGATGAGAAGCGAGCGGCTAACGCT
  CTCCAGAAGGAGAACGAGCAGAATCATCACGGAAATTCCGCTCTGATCGAGTTGGAGGGCGATAATGCAA
  GGCTGGCGGTATTGAAAAGGAATATCGAAGTGACCCACTTTGCGTACCCAGCTGTCAATTTGCCTCCCAA
  GGTGATGAGTGCAGTCATGAACCAGTTGCTCATTAAGCGCGCCCAGCCCATCGACAAGGATGCTAATCTC
  CAGGACCCAGAAGCCACGGATGACGGCAAACCAGTTGTGTCAGATGAGCAGTTGACCAAATGGCTCGGCA
  CCGATAATTCTAACGAACTGCAGCAGAGGAGAAAGCTCATGATGGCTGCCGTCCTGGTGACTGTGGAGCT
  GGAGTGCATGCATCGGTTTTTCTCCGATATCACAACCCTCCGAAAAATCGAGGAGTGTCTGCACTACACA
  TTTAGGCATGGTTACGTGCGGCAAGCATGCAAAATCTCTAACGTCGAGCTGTCAAATTTGGTCTCTTATA
  TGGGGATCCTTCATGAAAACAGACTGGGACAGAACGTCCTGCATTCTACTCTCCGCGATGAGGCCAGGAG
  AGACTATGTGAGAGACTGTATCTATTTGTTCCTGCTCCACACATGGCAGACCGGCATGGGGGTGTGGCAG
  CAGTGTCTGGAGGAAAAGAACCTGCGAGAACTCAATAAATTGCTGGACAGGGCTCTGAAAAGCCTTTGGA
  CTGGATTTGACGAGAGGACCGTTGCAGCCGAATTGGCCGACATTATCTTCCCCGAACGCCTCATGATCAC
  TCTGCAGAATGGACTCCCCGACTTCATGTCTCAGAGCATGTTGCACAATTACCGGAGCTTCATCTTGGAG
  AGATCAGGGATGTTGCCATCTATGTGTTGCGCCCTGCCATCTGATTTTGTCCCTATTTACTTCAGGGAGT
  GTCCTCCACCATTGTGGAGTCATTGCTACCTTCTGCGACTGGCTAATTACCTGGCTTACCACAGTGATCT
  CATGACTGATTCCTCCGGGGAAGGACTCATGGAGTGCCACTGCCGCTGCAATCTGTGCACGCCCCACAGG
  AGTCTGGTGTGTAATACCGAATTGCTCTCCGAGTCTCAAGTGATAGGCACCTTCGAGATGCAAGGACCGC
  AGTCTGATTCAAACTTCACAACAAACCTTAGACTGACACCCGGCTTGTGGACCAGTGCATATCTTCGGAA
  ATTCGAGCCACAGGATTACCACGCCCACTCCATAAATTTTTACGAGGATCAGAGCAAGCCTCCAAAAGCG
  CCACTGACAGCCTGCGTCATTACCCAGGGGAAGATCCTTGCTCAGTTGCACGCCATCAAGCAGGCTAGAG
  AGGAGTTTCTGCTGAAAAAGGGTCATGGAGTGTACCTCGACCCACAAACAGGCGAAGAGCTGAACTTGCC
  ATCCCCTCTGTGTGCCACAGCCAGCCCACACTCTCAGCACGTGCCAGAATCTCGCAAGACTGGTTACTGC
  GCTGCCACCCTTAAAGAGACCGCGGCTACTGCCGGCAACTTGGGCGGGAGAATCCTGGGGGAATCAGGAC
  GGGGCAGAGGTAGAGGACTTGGAAGGATGGGCGGGGGGGGTGGCGGTCAACCAAGAAGAGGGAGTCGCGG
  AGGCGGCGGCCGATTTCAGGGCAGAAGTGATAGGAGACAGACGGTGGCCTTCAACCAGGCCCTGTCTAAC
  GAAACTAGGTGCGAACAGATTAGCGAGAGTCAGCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100236 representing AP_000124
  Red=Cloning sites Green=Tags
  
  MMDLEPQESLTAPTAPAIGATAVMEKDKSLLIPQDAPVEQNLGYETPPEEFEGFLQIQKQPNEQNAGLED
  HDYLNEGDVLFKHLQRQSTIVRDAISDRSSIPVSIAELSCIYERNLFSPRVPPKRQANGTCEPNPRLNFY
  PVFAVPEALATYHIFFKNHKIPLSCRANRSRADELLALRAGASIPGIVSLEEVPKIFEGLGRDEKRAANA
  LQKENEQNHHGNSALIELEGDNARLAVLKRNIEVTHFAYPAVNLPPKVMSAVMNQLLIKRAQPIDKDANL
  QDPEATDDGKPVVSDEQLTKWLGTDNSNELQQRRKLMMAAVLVTVELECMHRFFSDITTLRKIEECLHYT
  FRHGYVRQACKISNVELSNLVSYMGILHENRLGQNVLHSTLRDEARRDYVRDCIYLFLLHTWQTGMGVWQ
  QCLEEKNLRELNKLLDRALKSLWTGFDERTVAAELADIIFPERLMITLQNGLPDFMSQSMLHNYRSFILE
  RSGMLPSMCCALPSDFVPIYFRECPPPLWSHCYLLRLANYLAYHSDLMTDSSGEGLMECHCRCNLCTPHR
  SLVCNTELLSESQVIGTFEMQGPQSDSNFTTNLRLTPGLWTSAYLRKFEPQDYHAHSINFYEDQSKPPKA
  PLTACVITQGKILAQLHAIKQAREEFLLKKGHGVYLDPQTGEELNLPSPLCATASPHSQHVPESRKTGYC
  AATLKETAATAGNLGGRILGESGRGRGRGLGRMGGGGGGQPRRGSRGGGGRFQGRSDRRQTVAFNQALSN
  ETRCEQISESQP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000005
• ORF Size: 2346 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000005.1, AP_000124
• RefSeq ORF: 2346
• MW: 87.7 kDa