protease (AC_000005) Virus Tagged ORF Clone

CAT#: VC100234

Myc-DDK-tagged ORF clone of viral ORF for protease [Human adenovirus A], codon optimized for human cell expression, AP_000122

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: protease
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100234 represents NCBI reference of AP_000122 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTTCTAGCGAGCAAGAGCTGACTGCCATTGTGAGAGACCTCGGCTGCGGGCCTTATTTTCTGGGTA
  CCTTTGATAAGCGCTTCCCAGGCTTCGTAAGCAGAGACAGATTGTCTTGTGCTATAGTGAATACAGCGGG
  TCGGGAAACCGGCGGGGTGCATTGGCTGGCCTTTGGGTGGAACCCCAAGAGTCATACTTGCTACCTTTTC
  GATCCATTCGGCTTCAGTGACCAGCGGTTGAAACAGATATATCAGTTTGAATATGAGAGTTTGCTGCGGA
  GATCAGCCCTTGCTGCCACAAAGGACCGCTGTGTGACCCTGGAAAAGAGCACCCAGACAGTCCAGGGTCC
  TTTTAGTGCCGCTTGCGGACTGTTCTGTTGCATGTTCCTGCACGCTTTTACCCATTGGCCTGATCACCCC
  ATGGACAAAAATCCTACTATGGATCTGCTGACCGGCGTACCTAACTGTATGCTGCAGTCACCTCAGGTCG
  TAGGCACCCTCCAGAGAAACCAGAACGAGCTGTATAAGTTTCTGAACAATCTCAGCCCTTACTTTCGGCA
  CAACCGCGAACGCATCGAGAAGGCGACATCCTTCACTAAAATGCAGAACGGCCTGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100234 representing AP_000122
  Red=Cloning sites Green=Tags
  
  MGSSEQELTAIVRDLGCGPYFLGTFDKRFPGFVSRDRLSCAIVNTAGRETGGVHWLAFGWNPKSHTCYLF
  DPFGFSDQRLKQIYQFEYESLLRRSALAATKDRCVTLEKSTQTVQGPFSAACGLFCCMFLHAFTHWPDHP
  MDKNPTMDLLTGVPNCMLQSPQVVGTLQRNQNELYKFLNNLSPYFRHNRERIEKATSFTKMQNGLK
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000005
• ORF Size: 618 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000005.1, AP_000122
• RefSeq ORF: 618
• MW: 23.5 kDa