U exon (AC_000005) Virus Tagged ORF Clone

CAT#: VC100247

Myc-DDK-tagged ORF clone of viral ORF for U exon, partial [Human adenovirus A], codon optimized for human cell expression, AP_000134

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U exon
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100247 represents NCBI reference of AP_000134 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATGGTCGGCCGGGATTGGGAGGAGGAGTCAGACCTGCCATTCAAGATCTGGCGAAAATTTGCCG
  TATGCAAGTCTGTTCGCTTCGACAGTTGGGAGGAGGGAAACTTTATAAGAGTGCACGGTGATCACGATGT
  GTTTTCCGATTTCCGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100247 representing AP_000134
  Red=Cloning sites Green=Tags
  
  MKMVGRDWEEESDLPFKIWRKFAVCKSVRFDSWEEGNFIRVHGDHDVFSDFR
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000005
• ORF Size: 156 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000005.1, AP_000134
• RefSeq ORF: 156
• MW: 6.4 kDa