E3 CR1-alpha1 (AC_000005) Virus Tagged ORF Clone

CAT#: VC100240

Myc-DDK-tagged ORF clone of viral ORF for E3 CR1-alpha1 [Human adenovirus A], codon optimized for human cell expression, AP_000129

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3 CR1-alpha1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100240 represents NCBI reference of AP_000129 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATCTCAGCAGTGATTTGCGTCCTTAATCTCATCATCTGCTCAGGAGCGGTGCCACCTGAAGAGG
  AGCCCAACTGTCACCCCCACCTGTCTAACATCAAGATCAATCTTAGTATCCCACATATTACCCTCAGATG
  CAGTTTTTTCTCTACACATCTGACGTGGACCTTCAACGGCAAGCATGTGACCAACACGGACATCAAATTT
  AAGTTGCACAAGGAGAACATCACTTTGTTCCAGCCTATCAACCTCGGCTACTACCGGTGTTCCGCTCCAC
  CCTGTACCCAAGCATTTTTTGTGGCTCCCGTCATCGACAAACGACCGGCTCCAACTACGGCGGCCGTGAC
  CGAGCACATTACGGAGGCCGTTAGCCCAAGCAAGGGGACCGAGGAGATCGTCTACTTCAGTAATTTTACC
  AACCATCTGGTTCTTAACTGTTCATGCAGTAATTCCCTTATTTCTTGGTTCGCGAATAGCAGCTTGTGTA
  AGACTTTCTATCAGGGCAAGCTTCTCTATAGCGCCAAGCTGACTCTGTGTAACCAGTCCACTCCCTCCCA
  CCTGACCCTCCTGCCCCCTTTCGTCGCCGGTCGCTACTTCTGTATCGGCGCCGCCCGAACCAGCCCTTGC
  CAACAGCATTGGAACCTCACATATTGTCCACCCCCTGTGTCACCATTCGTGATCAACACAGAGTACCTTG
  ATTACAATCCCCTGCTTGCTTACGGAGGTCTGGCCGCCTTGATTCTCTTTCTGATTTCTAATCTGTTCCT
  TGTGCAGCATTTGTACTCTTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100240 representing AP_000129
  Red=Cloning sites Green=Tags
  
  MKISAVICVLNLIICSGAVPPEEEPNCHPHLSNIKINLSIPHITLRCSFFSTHLTWTFNGKHVTNTDIKF
  KLHKENITLFQPINLGYYRCSAPPCTQAFFVAPVIDKRPAPTTAAVTEHITEAVSPSKGTEEIVYFSNFT
  NHLVLNCSCSNSLISWFANSSLCKTFYQGKLLYSAKLTLCNQSTPSHLTLLPPFVAGRYFCIGAARTSPC
  QQHWNLTYCPPPVSPFVINTEYLDYNPLLAYGGLAALILFLISNLFLVQHLYSY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000005
• ORF Size: 792 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000005.1, AP_000129
• RefSeq ORF: 792
• MW: 29.5 kDa