pVIII (AC_000005) Virus Tagged ORF Clone

CAT#: VC100245

Myc-DDK-tagged ORF clone of viral ORF for pVIII [Human adenovirus A], codon optimized for human cell expression, AP_000127

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pVIII
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100245 represents NCBI reference of AP_000127 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTAAGGACATTCCTACCCCGTATATGTGGTCTTTCCAGCCACAAATGGGACTGGCCGCCGGTGCGG
  CCCAAGATTACAGCTCCAAGATGAACTGGTTGTCCGCCGGGCCACATATGATCTCAAGAGTGAACGGCGT
  CAGAGCCAGAAGAAACCAGATCCTGCTGGAGCAGGCAGCCCTGACGGCTACTCCCAGAAATCAGCTCAAC
  CCACCTTCATGGCCAGCCGCACTGATCTATCAGGAAAACCCCCCCCCAACCACAGTTCTTCTGCCACGAG
  ATGCACAGGCTGAGGTACACATGACAAACGCCGGTGCTCAACTGGCCGGGGGAGCCCGGCATAGTTTCCG
  GTACAAGGGCAGAACCGAACCGTACCCGTCCCCTGCGATCAAAAGGGTTCTGATTAGGGGCAAGGGCATT
  CAGTTGAATGACGAGGTCACTTCCCCCCTCGGCGTAAGGCCGGACGGAGTGTTTCAGTTGGGAGGAAGCG
  GTAGATCAAGTTTTACCGCAAGGCAAGCATACCTCACTCTTCAGTCCTCTTCAAGCGCGCCCAGGAGCGG
  CGGGATCGGAACTCTTCAATTTGTGGAAGAGTTCACACCATCAGTCTACTTTAATCCATTCTCCGGAAGC
  CCTGGTCATTATCCCGATGCCTTCATCCCAAATTTTGACGCCGTGAGCGAGAGCGTGGACGGCTACGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100245 representing AP_000127
  Red=Cloning sites Green=Tags
  
  MSKDIPTPYMWSFQPQMGLAAGAAQDYSSKMNWLSAGPHMISRVNGVRARRNQILLEQAALTATPRNQLN
  PPSWPAALIYQENPPPTTVLLPRDAQAEVHMTNAGAQLAGGARHSFRYKGRTEPYPSPAIKRVLIRGKGI
  QLNDEVTSPLGVRPDGVFQLGGSGRSSFTARQAYLTLQSSSSAPRSGGIGTLQFVEEFTPSVYFNPFSGS
  PGHYPDAFIPNFDAVSESVDGYD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000005
• ORF Size: 699 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000005.1, AP_000127
• RefSeq ORF: 699
• MW: 25.3 kDa