E1A (NC_011202) Virus Tagged ORF Clone

CAT#: VC100290

Myc-DDK-tagged ORF clone of viral ORF for control protein E1A [Human adenovirus B], codon optimized for human cell expression, YP_002213798

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100290 represents NCBI reference of YP_002213798 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAGATTTGCGGTTCTTGCCGCAGGAGATTATTTCTGCAGAAACCGGCAATGAGATCCTGGAGCTGG
  TTGTGCACGCACTGATGGGAGATGACCCAGAGCCACCAGTGCAGCTCTTTGAACCCCCTACCCTTCAGGA
  ACTTTACGACCTCGAGGTAGAGGGTTCCGAGGATTCTAATGAAGAAGCGGTTAATGGGTTCTTCACCGAC
  AGCATGCTGCTTGCGGCAAATGAGGGACTGGAGTTGGACCCTCCTCTGGACACTTTTGATACGCCCGGCG
  TGATTGTTGAATCTGGCACCGGTGTGAGAAAACTGCCGGACCTCAGCTCTGTAGACTGCGATTTGCACTG
  CTATGAGGATGGCTTCCCACCTAGCGACGAGGAGGACCATGAGAAAGAACAAAGCATGCAGACAGCTGCG
  GGCGAAGGCGTTAAGGCCGCCAACGTAGGCTTCCAGCTGGACTGTCCCGAGCTCCCCGGGCACGGATGCA
  AGAGTTGCGAGTTTCACCGAAAAAATACGGGGGTAAAGGAACTGCTGTGTTCCTTGTGCTACATGCGGAC
  CCACTGCCACTTCATCTATTCTCCTGTTTCCGACGCGGATGAGAGCCCGTCCCCCGACAGCACCACAAGT
  CCCCCCGAGATTCAGGCACCTGTCCCCGTGGACGTTAGGAAGCCCATCCCTGTGAAATTGAAGCCAGGAA
  AGAGACCGGCCGTGGAAAAGCTTGAAGACCTCCTGCAGGGGGGTGATGGCCCGCTGGATCTCTCCACCCG
  AAAAAGGCCCCGACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100290 representing YP_002213798
  Red=Cloning sites Green=Tags
  
  MRDLRFLPQEIISAETGNEILELVVHALMGDDPEPPVQLFEPPTLQELYDLEVEGSEDSNEEAVNGFFTD
  SMLLAANEGLELDPPLDTFDTPGVIVESGTGVRKLPDLSSVDCDLHCYEDGFPPSDEEDHEKEQSMQTAA
  GEGVKAANVGFQLDCPELPGHGCKSCEFHRKNTGVKELLCSLCYMRTHCHFIYSPVSDADESPSPDSTTS
  PPEIQAPVPVDVRKPIPVKLKPGKRPAVEKLEDLLQGGDGPLDLSTRKRPRQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011202
• ORF Size: 786 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011202.1, YP_002213798
• RefSeq ORF: 786
• Locus ID: 6870514
• MW: 28.7 kDa