E1B (NC_011202) Virus Tagged ORF Clone

CAT#: VC100291

Myc-DDK-tagged ORF clone of viral ORF for control protein E1B 19K [Human adenovirus B], codon optimized for human cell expression, YP_002213799

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100291 represents NCBI reference of YP_002213799 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGTGTGGGCAATCCTTGAGGACCTCAGGAAGACACGGCAGCTGCTCGAAAACGCCAGTGACGGCG
  TGTCTGGCTTCTGGCGGTTTTGGTTTGCGTCCGAACTCGCCCGGGTCGTTTTTAGGATCAAGCAGGACTA
  TAATCAGGAGTTTGAAAAACTGTTGGTGGATTGCCCTGGCCTGTTCGAGGCACTCAACTTGGGTCATCAA
  GTGCATTTTAAGGAAAAGGTCCTCTCTGTCCTCGACTTTTCTACTCCAGGAAGAACAGCCGCCGCAGTGG
  CGTTTCTGACTTTTATACTTGACAAGTGGATCCCCCAAACTCATTTTTCCCGAGGCTACGTCTTGGACTT
  CATTGCTACGGCGCTCTGGCGAACGTGGAAAGTCAGAAAAATGCGCACAATCCTCGGGTATTGGCCCGTC
  CAGCCCTTGGGAGTTGCAGGCATCCTCAGGCATCCACCTGTCATGCCAGCCGTGCTCGAGGAAGAGCAGC
  AAGAGGACAATCCGCGAGCAGGACTCGATCCACCAGTGGAAGAGGCAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100291 representing YP_002213799
  Red=Cloning sites Green=Tags
  
  MEVWAILEDLRKTRQLLENASDGVSGFWRFWFASELARVVFRIKQDYNQEFEKLLVDCPGLFEALNLGHQ
  VHFKEKVLSVLDFSTPGRTAAAVAFLTFILDKWIPQTHFSRGYVLDFIATALWRTWKVRKMRTILGYWPV
  QPLGVAGILRHPPVMPAVLEEEQQEDNPRAGLDPPVEEAE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011202
• ORF Size: 540 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011202.1, YP_002213799
• RefSeq ORF: 540
• Locus ID: 6870499
• MW: 20.8 kDa