L4 (NC_011202) Virus Tagged ORF Clone

CAT#: VC100298

Myc-DDK-tagged ORF clone of viral ORF for capsid protein precursor pVIII [Human adenovirus B], codon optimized for human cell expression, YP_002213818

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100298 represents NCBI reference of YP_002213818 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAAGGAGATTCCGACCCCATACATGTGGTCCTACCAGCCTCAGATGGGCCTTGCCGCCGGAGCTT
  CACAGGACTACAGTACCCGGATGAATTGGCTGTCCGCCGGGCCGTCAATGATTTCTCGGGTGAACGATAT
  TCGAGCCTATCGGAACCAGATTCTGCTCGAGCAATCAGCACTGACAACCACACCTCGGCAGCACCTGAAT
  CCCCGCAATTGGCCAGCTGCTCTGGTGTACCAAGAATCACCCGCGCCCACAACAGTCTTGTTGCCTCGGG
  ATGCACAAGCTGAAGTCCAAATGACCAATGCCGGCGCCCAACTGGCAGGAGGATCTACCTTGTGCCGGCA
  CCGCCCCCGACATAATATCAAACGGCTCATGATCAGGGGGCGCGGGATACAGCTCAACGATGAGTCCGTA
  TCTTCACCCCTCGGTCTGCGACCAGACGGAATCTTCCAAATCGCTGGCTGCGGAAGGAGTTCCTTCACAC
  CCAGACAAGCTGTGCTTACGCTCGAGAGCTCTAGCAGTCAACCTCGGTCCGGCGGGATAGGCACCGTCCA
  GTTCGTCGAAGAATTCACGCCATCTGTGTACTTCAACCCGTTCAGTGGGTCCCCCGGGCATTACCCGGAT
  GAGTTTATCCCAAATTTCGATGCAATTAGCGAAAGCGTAGACGGATATGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100298 representing YP_002213818
  Red=Cloning sites Green=Tags
  
  MSKEIPTPYMWSYQPQMGLAAGASQDYSTRMNWLSAGPSMISRVNDIRAYRNQILLEQSALTTTPRQHLN
  PRNWPAALVYQESPAPTTVLLPRDAQAEVQMTNAGAQLAGGSTLCRHRPRHNIKRLMIRGRGIQLNDESV
  SSPLGLRPDGIFQIAGCGRSSFTPRQAVLTLESSSSQPRSGGIGTVQFVEEFTPSVYFNPFSGSPGHYPD
  EFIPNFDAISESVDGYD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011202
• ORF Size: 681 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011202.1, YP_002213818
• RefSeq ORF: 681
• Locus ID: 6870504
• MW: 25.0 kDa