IVa2 (NC_011202) Virus Tagged ORF Clone

CAT#: VC100296

Myc-DDK-tagged ORF clone of viral ORF for encapsidation protein IVa2 [Human adenovirus B], codon optimized for human cell expression, YP_002213841

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IVa2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100296 represents NCBI reference of YP_002213841 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACCAAGGGGCGCCAGGCAACTGAAGTTTTTGATCAGCAAGATGAATCAGAAGCCCACCCTGGCA
  AGAGACCTTCTCGGCGCACACCTCTGCATTGCGATGGAAACAATCCAGACTCCAATACTGAAACGCTGGA
  AGGCCCCAATCCCGGCTCCGCCGGAAGACCATCCAGCCACACACTGTTCCAAGAGCCATCAAAGCCCGCC
  AAGCGGGGGGGCCTCCTCGATCGCGATGCGATTGAGCACATCACTGAGCTTTGGAACCGGCTGGAGCTGC
  TGCAGCAAACTCTGTCAAAGATGCCAATGGCAGACGGGTTGAAACCACTCAAAAATTTTTCTTCACTGCA
  AGAGCTGCTGAGTCTCGGTGGAGAACGACTGCTCATGGATCTGGTGAGAGAGAACATGCACGTCCGGGAA
  ATGATGAATGAAGTGGCCCCACTCCTCCGGGAGGACGGTAGTTGCAGAAGCCTCAACTATCATCTGCAAC
  CCGTGATCGGGATCATCTATGGCCCTACTGGATGCGGAAAGTCCCAGCTGCTGAGAAACCTGCTTTCCTC
  ACAACTTATCACCCCTGCTCCCGAGACGGTATTCTTCATTGCCCCACAGGTAGACATGATCCCACCTAGC
  GAATTGAAGGCTTGGGAAATGCAGATCTGCGAAGGCAATTACGCGCCTGGACCAGAGGGAACATTCATCC
  CGCAGTCTGGGACTCTCAGACCTAAGTTTATAAAAATGGCTTATGACGACCTCACCCAGGAACACAATTA
  CGATGTCTCCGACCCTAGAAATGTCTTCGCCCGGGCCGCAGCTCATGGCCCCATTGCCATCATAATGGAT
  GAATGCATGGAAAACCTGGGGGGGCACAAGGGTGTGAGCAAATTCTTTCATGCTTTTCCTTCTAAGCTGC
  ATGATAAGTTTCCAAAGTGTACTGGGTACACCGTGTTGGTTGTGCTGCACAACATGAACCCAAGACGCGA
  CCTGGGAGGAAACATTGCCAATTTGAAGATTCAAAGCAAGATGCACATCATAAGCCCACGAATGCATCCC
  TCACAGTTGAATAGATTTGTGAACACATACACCAAGGGCCTCTCAGTTGCCATTTCACTCCTCCTTAAAG
  ACATCGTTCAGCACCACGCACTCAGGCCATGCTATGACTGGGTTATTTACAATACGACCCCTGAACACGA
  ATCCCTGCAGTGGAGTTATCTGCACCCCAAAGATGGCCTCATGCCCATGTACTTGAACATCCAAAGCCAC
  CTCTACAGAATCTTGGAGAAAATTCACCGGGTGCTGAACGACCGGGACCGATGGAGTAGAGCCTATCGAG
  CTCGCAAAATGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100296 representing YP_002213841
  Red=Cloning sites Green=Tags
  
  METKGRQATEVFDQQDESEAHPGKRPSRRTPLHCDGNNPDSNTETLEGPNPGSAGRPSSHTLFQEPSKPA
  KRGGLLDRDAIEHITELWNRLELLQQTLSKMPMADGLKPLKNFSSLQELLSLGGERLLMDLVRENMHVRE
  MMNEVAPLLREDGSCRSLNYHLQPVIGIIYGPTGCGKSQLLRNLLSSQLITPAPETVFFIAPQVDMIPPS
  ELKAWEMQICEGNYAPGPEGTFIPQSGTLRPKFIKMAYDDLTQEHNYDVSDPRNVFARAAAHGPIAIIMD
  ECMENLGGHKGVSKFFHAFPSKLHDKFPKCTGYTVLVVLHNMNPRRDLGGNIANLKIQSKMHIISPRMHP
  SQLNRFVNTYTKGLSVAISLLLKDIVQHHALRPCYDWVIYNTTPEHESLQWSYLHPKDGLMPMYLNIQSH
  LYRILEKIHRVLNDRDRWSRAYRARKMK
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011202
• ORF Size: 1344 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011202.1, YP_002213841
• RefSeq ORF: 1344
• Locus ID: 6870502
• MW: 50.9 kDa