L2 (NC_011202) Virus Tagged ORF Clone

CAT#: VC100306

Myc-DDK-tagged ORF clone of viral ORF for core protein V [Human adenovirus B], codon optimized for human cell expression, YP_002213809

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100306 represents NCBI reference of YP_002213809 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAAACGCAAATATAAGGAGGAGATGCTGCAGGTGATTGCGCCTGAGGTATATGGCCAGCCCCTGA
  AGGACGAGAAGAAACCACGCAAGATTAAGAGAGTGAAGAAGGATAAGAAGGAGGAGGAGGACGGCGACGA
  TGGACTTGCTGAATTTGTGCGAGAGTTCGCCCCCAGAAGGCGCGTGCAATGGCGCGGTCGAAAGGTGAGG
  CACGTTCTGCGCCCCGGTACTTCTGTTGTCTTCACGCCTGGGGAGAGGAGCTCCGCCACCTTTAAACGAT
  CCTATGACGAAGTGTACGGCGACGACGATATTCTCGAGCAGGCCGCAGACCGCCTGGGTGAATTTGCTTA
  CGGCAAGCGGAGCAGGATTACAAGTAAGGACGAAACAGTCAGCATCCCTCTGGATCATGGAAACCCCACT
  CCCTCTCTGAAGCCCGTCACGCTGCAACAGGTGCTGCCTGTGACGCCAAGGACAGGCGTGAAGCGGGAAG
  GCGAGGATCTGTACCCCACCATGCAACTCATGGTGCCTAAAAGGCAGAAGCTGGAGGACGTGCTGGAGAA
  AGTTAAGGTCGATCCGGATATCCAGCCCGAGGTGAAAGTACGGCCCATTAAACAGGTAGCACCCGGTCTC
  GGGGTCCAAACCGTAGACATAAAAATCCCAACAGAATCCATGGAAGTGCAAACCGAGCCCGCGAAGCCAA
  CAGCTACCTCCACCGAGGTGCAAACCGACCCGTGGATGCCGATGCCTATAACCACCGATGCAGCAGGCCC
  TACTCGCAGAAGCCGGAGAAAATATGGCCCTGCATCTCTGCTTATGCCGAACTACGTTGTTCACCCCAGC
  ATCATTCCGACACCAGGTTACAGAGGGACCAGGTACTACAGGTCAAGAAACAGCACATCTCGCAGGAGAA
  GGAAGACCCCCGCAAATAGGTCCAGAAGACGGCGAAGAACTTCAAAGCCCACACCTGGAGCGTTGGTAAG
  GCAAGTCTACCGGAATGGATCCGCTGAACCCCTCACGTTGCCTAGAGCTAGATACCATCCATCCATCATA
  ACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100306 representing YP_002213809
  Red=Cloning sites Green=Tags
  
  MSKRKYKEEMLQVIAPEVYGQPLKDEKKPRKIKRVKKDKKEEEDGDDGLAEFVREFAPRRRVQWRGRKVR
  HVLRPGTSVVFTPGERSSATFKRSYDEVYGDDDILEQAADRLGEFAYGKRSRITSKDETVSIPLDHGNPT
  PSLKPVTLQQVLPVTPRTGVKREGEDLYPTMQLMVPKRQKLEDVLEKVKVDPDIQPEVKVRPIKQVAPGL
  GVQTVDIKIPTESMEVQTEPAKPTATSTEVQTDPWMPMPITTDAAGPTRRSRRKYGPASLLMPNYVVHPS
  IIPTPGYRGTRYYRSRNSTSRRRRKTPANRSRRRRRTSKPTPGALVRQVYRNGSAEPLTLPRARYHPSII
  T
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011202
• ORF Size: 1053 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011202.1, YP_002213809
• RefSeq ORF: 1053
• Locus ID: 6870506
• MW: 40.1 kDa