E2B (NC_011202) Virus Tagged ORF Clone

CAT#: VC100294

Myc-DDK-tagged ORF clone of viral ORF for DNA polymerase [Human adenovirus B], codon optimized for human cell expression, YP_002213842

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100294 represents NCBI reference of YP_002213842 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATTGGTGCAGGCTCACGGCGCGGGGTCCTTTCACCCAGAGACCTCCGACACAAGGAGGCAGTCCA
  CTCGGGGTGGCGCTAGGAAGAGCTCACAGAGCGCTGTGCCCGAACCAGCCCGCGCACGGAGGAGAAGGGC
  TACCACTAGCCACGCATTGGGAAGCGGTACAACTGCCGCTGCCAGAAGGGCATCCCCTTCACCACTGCTT
  TCCATGCAGGAGGCGAGAGAACCCCCCCCAATTAAAAGAAAGAACAAGGGAACTGTGGTCGCCCCCAAGG
  GTCACGGCACACTGCAGGCGATAGACGTGAGTACCAATGAGCCAGTCGAGATCAAATACCACCTGAACTT
  GCCCCACGCTCTGGAGAAGATCATGCAGGTCAACTTGTTGTCCCTGCCCACTAACCTTTCCCCTCAGCGC
  CTGAGGACCCTGGACTCCAGTAGCCTGAGAGCCTTGGTTCTGCAGCTTAGACCATCTCGAGCCGAGGTTT
  GGACCTGCCTGCCCAGAGGGCTCGTCTCTATGACGACTATCGAGACTGAAGACGAGCAAGCAGATACCGC
  CAATATCGAGGAACACGAAATTCAAAGCCCCGGTCTGGGTTTTCCTCTCAAATTTCTCGTCAAGGGTAGC
  CAAGTGCAGCTGATTCACGAAATTCAGCCGGTGCAGAGATGCGACTATTGCGGAAGACTGTATAAACACA
  AGCACGAATGCAGCGCCCGCCGGCGAAACTTCTATTTCCATCACATTAATAGCCAATCATCTAACTGGTG
  GCAGGAGATACAGTTTTTTCCCATCGGATCCCACCCAAGAACCGAACGCCTGTTCCTGACCTACGATGTG
  GAGACCTACACCTGGATGGGATCATTTGGGAAGCAGCTCGTGCCTTTTATGCTTGTCATGAAGTTGTCAG
  GGGAGGAGAGATTGGTGAATATGGCCCATGACCTGGCTATAAAACTGAAATGGGATCGATGGCAGCAGGA
  TCCCAGAACCTTTTACTGCGTTACACCCGAGAAAATGGCTATTGGGCGCCATTTTAGGCAGTACCGAGAC
  CAACTGCAGACGGCCCTTGCAGTGGATCTCTGGTCAAGTTTTCTGCACGCGAACCCCCATCTGCATGAAT
  GGGCACTGGAGCATTACGCACTGAGCGATCCTACCGATCTCACTTTTGACGAACTGAGCAAGCTTCCGCA
  TGTGCGAGGCACCCCCCGATTCATTGAACTGTACATCGTTGGACATAACATTAACGGATTTGACGAGATC
  GTTTTGGCCGCCCAGGTGATTAACAACCGGGCCGAAGTGCCACAGCCTTTCAGGATCACCAGGAATTTCA
  TGCCTAGAGCCGGGAAGATCCTTTTTAATGACGTAACATTTGCTCTCCCAAACCCTGCCTACAAGAAGAG
  AGTTGATTTCCAGCTGTGGGAGCAAGGAGCTTGTGATGATATCGATTTCAAATATCAATTTCTGAAAGTG
  ATGGTTAGAGACACTTTCGCCCTCACACATGCTTCCCTTCGGAAAGCCGCCCAAGCCTACTCCCTGCCCG
  TGGAGAAAGGTTGCTGTCCTTATAAAGCAGTGAATCAGTTCTACATGCTGGGGAGCTACCGCGCTGACAA
  AGACGGTTTTCCCCTCGAGGAATATTGGAAGGACCGGGAGGAGTACCTGCTGAACCGGGAGTTGTGGGAG
  AAAAAAAATCAACCTGGCTATGATATTATACAGGAGACCTTGGACTACTGCGCCCTGGACGTCCTGGTGA
  CGGCGGAACTCGTTACGAAGCTGCAAGAGTCATACGCCCATTTTGTCCGGGACTCCGTCGGCTTGCCTCA
  CGCTCATTTTAACATATTTCAGCGCCCCACTATCAGCTCAAACAGTCATGCAATATTTCGACAAATAGTG
  TATCGAACTGAGAAGCCAAACCGCACCAACCTCGGCCCGGGACTGCTCGCTCCCTCTCATGAGTTGTACG
  ATTACGTCCGGGCCTCAATACGCGGCGGGAGATGTTATCCCACCTACATTGGCATCTTTGAGGATCCGAT
  ATACGTGTATGATATCTGTGGTATGTATGCCTCCGCACTCACGCACCCAATGCCTTGGGGCAGCCCGCTG
  AATCCTTATGAGAGAGCCCTGGCCGCAAGAGAGTGGCAGATGGCTCTTGATGACCCGACACCAATCTCAT
  ATTTCGACAAAGACCTTCTCCCTGGCATCTTCACAATGGACGCTGATCCCCCAGATGAGTTGATGCTTGA
  CCCCTTGCCCCCATTCTGTTCCCGCAAGGGAGGACGGCTGTGCTGGACTAATGAGCCACTGCGGGGCGAA
  GTAGCCACCTCAGTCGACCTGATCACACTGCACAACAGGGGTTGGCAAGTGAGGATCGTGCCTGACGAAC
  TCACGACCATATTCCCAGAGTGGAAATGCCTGGCCAGAGAGTACGTGCAGTTGAATATCGCAGCTAAAGA
  ACGCGCTGACAAAGAGAAAAACCAGACAATGCGCTCAATCGCGAAACTGCTGAGTAATGCACTGTATGGG
  AGCTTTGCTACTAAACTGGATAACAAAAAGATTGTGTTTTCAGACCAGATGGATGAAAACCTCATGAAGG
  GCATTTCAACCGGCACCGTAAACATTAAGTCAAGTTCTTTTTTGGAAACCGATAATTTGAGTGCCGAAGT
  TATGCCAGCATTCGAGCGGGAGTACCTCCCCCAACAGCTGACGTTTCTTGACTCCGACCCTGAAGACAGC
  GAGGAGGAGCAACGAACCGCCCCATTTTATACGCCCCCCGCCGGGACCCCTGGCCACGTGAAGTACACTT
  ATAAGCCTATTACATTCCTCGACGTGGAGGAAGGTGACATGTGTCTGCATACTTTGGAGAAGGTCGACCC
  GCTTGTGGATAATGACAGGTACCCTTCTCATATTGCGAGCTTTGTGCTCGCGTGGACTAGGGCTTTCGTG
  AGTGAGTGGAGTGAGTTTTTGTATGAAGAAGACCGAGGGACACCCCTCGAAAAGAGACCTGTGAAGAGCG
  TGTACGGGGATACTGACTCACTCTTCGTTACCCAGCGCGGACACGAACTTATGGAAACTAAAGGCAAGAA
  GAGAATCAAGAAGTACGGCGGTAAGCTGGTCTTCGACCCCAACGAACCCGATCTGACCTGGCTGGTGGAG
  TGCGAAACGGTGTGCGCGAGCTGCGGTGCCGACGCATACTCTCCCGAATCCGTCTTCCTCGCTCCTAAAC
  TGTATGCTCTCAAGTGCCTGTATTGTCCTGTTTGTAAGAAGACTTCTAAGGGTAAACTGAGGGCCAAAGG
  CCATGCTGCCGAGGCTTTGAATTACGAACTGATGGTTAACTGTTACCTGGCCGACATCCAGGGGGCCAAG
  CGACAAAAGTTTAGTACATCTAGGATGAGCCTGAAGCGAACCCTGGCATCCGCTCAAGCCGGCGCTCACC
  CCTTTACGGTGACCGAAACGACACTGACCAGAACACTGAGACCATGGAAGGATCGGACTCTGGCTGCACT
  CGACGCACACCGACTTATCCCTTACTCCAGGAGTAGACCAAACCCGCGCAATGAGGAGGTCTGCTGGATC
  GAAATGCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100294 representing YP_002213842
  Red=Cloning sites Green=Tags
  
  MALVQAHGAGSFHPETSDTRRQSTRGGARKSSQSAVPEPARARRRRATTSHALGSGTTAAARRASPSPLL
  SMQEAREPPPIKRKNKGTVVAPKGHGTLQAIDVSTNEPVEIKYHLNLPHALEKIMQVNLLSLPTNLSPQR
  LRTLDSSSLRALVLQLRPSRAEVWTCLPRGLVSMTTIETEDEQADTANIEEHEIQSPGLGFPLKFLVKGS
  QVQLIHEIQPVQRCDYCGRLYKHKHECSARRRNFYFHHINSQSSNWWQEIQFFPIGSHPRTERLFLTYDV
  ETYTWMGSFGKQLVPFMLVMKLSGEERLVNMAHDLAIKLKWDRWQQDPRTFYCVTPEKMAIGRHFRQYRD
  QLQTALAVDLWSSFLHANPHLHEWALEHYALSDPTDLTFDELSKLPHVRGTPRFIELYIVGHNINGFDEI
  VLAAQVINNRAEVPQPFRITRNFMPRAGKILFNDVTFALPNPAYKKRVDFQLWEQGACDDIDFKYQFLKV
  MVRDTFALTHASLRKAAQAYSLPVEKGCCPYKAVNQFYMLGSYRADKDGFPLEEYWKDREEYLLNRELWE
  KKNQPGYDIIQETLDYCALDVLVTAELVTKLQESYAHFVRDSVGLPHAHFNIFQRPTISSNSHAIFRQIV
  YRTEKPNRTNLGPGLLAPSHELYDYVRASIRGGRCYPTYIGIFEDPIYVYDICGMYASALTHPMPWGSPL
  NPYERALAAREWQMALDDPTPISYFDKDLLPGIFTMDADPPDELMLDPLPPFCSRKGGRLCWTNEPLRGE
  VATSVDLITLHNRGWQVRIVPDELTTIFPEWKCLAREYVQLNIAAKERADKEKNQTMRSIAKLLSNALYG
  SFATKLDNKKIVFSDQMDENLMKGISTGTVNIKSSSFLETDNLSAEVMPAFEREYLPQQLTFLDSDPEDS
  EEEQRTAPFYTPPAGTPGHVKYTYKPITFLDVEEGDMCLHTLEKVDPLVDNDRYPSHIASFVLAWTRAFV
  SEWSEFLYEEDRGTPLEKRPVKSVYGDTDSLFVTQRGHELMETKGKKRIKKYGGKLVFDPNEPDLTWLVE
  CETVCASCGADAYSPESVFLAPKLYALKCLYCPVCKKTSKGKLRAKGHAAEALNYELMVNCYLADIQGAK
  RQKFSTSRMSLKRTLASAQAGAHPFTVTETTLTRTLRPWKDRTLAALDAHRLIPYSRSRPNPRNEEVCWI
  EMP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011202
• ORF Size: 3579 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011202.1, YP_002213842
• RefSeq ORF: 3579
• Locus ID: 6870501
• MW: 136.2 kDa