L3 (NC_011202) Virus Tagged ORF Clone

CAT#: VC100303

Myc-DDK-tagged ORF clone of viral ORF for hexon [Human adenovirus B], codon optimized for human cell expression, YP_002213812

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100303 represents NCBI reference of YP_002213812 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAACCCCCTCCATGTTGCCGCAGTGGGCATACATGCACATCGCCGGACAAGACGCTAGCGAATACT
  TGAGTCCCGGACTCGTTCAGTTCGCACGGGCAACAGACACATATTTTAACCTGGGAAACAAATTTAGGAA
  TCCTACGGTTGCACCGACACATGACGTCACAACCGACAGGTCCCAACGATTGATGCTTCGGTTCGTACCA
  GTTGATAGGGAAGATAACACCTATAGTTATAAGGTGCGCTACACTCTTGCCGTTGGAGATAATAGGGTGC
  TGGACATGGCTTCTACATTCTTTGATATTAGGGGTGTGCTGGACAGAGGGCCAAGCTTCAAACCGTACTC
  AGGCACAGCCTATAACAGTTTGGCTCCTAAAGGGGCTCCAAATACATCACAGTGGATTGCCGAGGGAGTA
  AAAAATACAACTGGTGAAGAGCATGTTACGGAGGAGGAGACCAACACTACAACCTACACGTTCGGCAATG
  CCCCCGTCAAAGCCGAGGCCGAGATCACTAAGGAGGGTCTCCCTGTGGGCCTTGAAGTGTCAGACGAGGA
  GAGCAAGCCCATTTACGCAGACAAAACTTACCAACCAGAACCCCAACTGGGTGACGAGACATGGACTGAT
  CTGGATGGCAAGACCGAGAAATACGGCGGAAGGGCTTTGAAACCCGACACCAAGATGAAGCCATGTTATG
  GGTCCTTCGCGAAACCTACCAATGTCAAAGGGGGGCAGGCAAAGCAGAAGACCACGGAGCAGCCCAATCA
  GAAGGTCGAATATGACATAGATATGGAATTTTTCGACGCAGCCAGCCAGAAGACTAACCTGTCACCTAAG
  ATTGTTATGTATGCAGAGAACGTGAACCTGGAAACGCCGGACACCCATGTGGTCTATAAGCCCGGTACAG
  AAGATACATCTTCTGAGGCCAACCTGGGTCAACAGAGTATGCCTAACAGACCAAATTACATCGGCTTCCG
  CGACAATTTTATTGGGTTGATGTATTATAATAGCACCGGTAACATGGGGGTTCTTGCCGGTCAGGCATCA
  CAGCTGAACGCCGTAGTAGATCTCCAGGATAGAAATACCGAGCTGTCATACCAGCTCTTGCTGGATAGTT
  TGGGCGACCGCACACGCTACTTTAGTATGTGGAACCAGGCAGTCGATTCCTATGACCCAGATGTGCGGGT
  GATAGAGAACCATGGCGTAGAGGACGAGCTGCCAAATTACTGTTTTCCTCTGGATGGCATTGGTGTGCCT
  ACGACATCTTACAAGTCCATTGTCCCCAATGGTGATAACGCCCCCAATTGGAAGGAGCCCGAGGTTAACG
  GCACATCAGAAATCGGCCAGGGCAATCTTTTTGCCATGGAGATTAATCTCCAGGCCAACCTTTGGCGCAG
  CTTCCTGTATTCAAATGTTGCACTGTACCTCCCTGATAGTTATAAGTACACCCCATCCAATGTGACTCTC
  CCCGAAAACAAGAATACATACGATTATATGAATGGCCGGGTCGTTCCTCCTTCCCTTGTTGACACATACG
  TAAATATTGGCGCACGGTGGAGCCTTGACGCGATGGACAATGTCAACCCTTTCAACCACCATAGGAACGC
  TGGTTTGCGCTATCGGTCCATGCTGCTGGGAAACGGGCGATATGTGCCGTTCCACATTCAAGTGCCCCAG
  AAGTTTTTTGCCGTCAAGAATCTGCTTCTGTTGCCCGGCTCTTATACTTATGAGTGGAACTTCCGGAAGG
  ACGTGAACATGGTTCTTCAGTCCTCTTTGGGCAATGATCTTAGAGTGGACGGCGCCAGCATATCTTTTAC
  CAGCATCAACCTCTATGCGACCTTTTTTCCTATGGCTCATAACACAGCTAGCACATTGGAGGCCATGCTG
  CGCAATGATACAAACGACCAGTCATTTAACGACTATTTGTCCGCTGCTAATATGTTGTACCCCATTCCTG
  CAAACGCAACCAATATTCCAATCTCTATTCCAAGCCGCAACTGGGCCGCCTTTCGGGGTTGGAGCTTTAC
  ACGGCTGAAGACTAAGGAAACTCCGTCCCTCGGGAGCGGCTTCGACCCTTATTTCGTTTATAGCGGATCA
  ATCCCGTACCTGGATGGAACCTTTTATCTCAACCACACCTTCAAAAAGGTCTCCATCATGTTTGACAGCA
  GTGTGAGTTGGCCCGGAAATGATAGACTCCTTTCACCCAACGAGTTTGAAATTAAACGCACAGTCGACGG
  AGAGGGCTATAACGTTGCGCAGTGCAACATGACTAAGGATTGGTTCCTGGTACAGATGTTGGCAAATTAT
  AACATCGGATACCAGGGCTTTTATATTCCTGAAGGGTATAAGGATAGAATGTATTCTTTCTTTCGGAATT
  TCCAGCCAATGTCACGGCAGGTCGTGGACGAGGTGAACTATAAAGACTTTAAAGCCGTCGCAATTCCCTA
  CCAGCATAATAACTCCGGGTTTGTGGGGTACATGGCTCCAACTATGCGGCAGGGGCAGCCGTACCCTGCA
  AACTATCCCTACCCCCTTATCGGAACCACTGCCGTGAACTCAGTGACACAGAAAAAGTTCCTCTGCGACA
  GAACCATGTGGCGAATCCCATTCTCTAGCAACTTTATGTCTATGGGCGCACTGACAGATCTGGGCCAGAA
  CATGTTGTACGCGAACTCAGCACACGCATTGGATATGACTTTTGAAGTGGACCCCATGGATGAACCCACA
  CTCCTGTATCTTCTCTTTGAGGTCTTTGATGTGGTGAGGGTGCATCAACCACACCGCGGCATAATCGAAG
  CCGTGTATCTCCGGACACCTTTCTCAGCAGGCAATGCAACAACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100303 representing YP_002213812
  Red=Cloning sites Green=Tags
  
  MATPSMLPQWAYMHIAGQDASEYLSPGLVQFARATDTYFNLGNKFRNPTVAPTHDVTTDRSQRLMLRFVP
  VDREDNTYSYKVRYTLAVGDNRVLDMASTFFDIRGVLDRGPSFKPYSGTAYNSLAPKGAPNTSQWIAEGV
  KNTTGEEHVTEEETNTTTYTFGNAPVKAEAEITKEGLPVGLEVSDEESKPIYADKTYQPEPQLGDETWTD
  LDGKTEKYGGRALKPDTKMKPCYGSFAKPTNVKGGQAKQKTTEQPNQKVEYDIDMEFFDAASQKTNLSPK
  IVMYAENVNLETPDTHVVYKPGTEDTSSEANLGQQSMPNRPNYIGFRDNFIGLMYYNSTGNMGVLAGQAS
  QLNAVVDLQDRNTELSYQLLLDSLGDRTRYFSMWNQAVDSYDPDVRVIENHGVEDELPNYCFPLDGIGVP
  TTSYKSIVPNGDNAPNWKEPEVNGTSEIGQGNLFAMEINLQANLWRSFLYSNVALYLPDSYKYTPSNVTL
  PENKNTYDYMNGRVVPPSLVDTYVNIGARWSLDAMDNVNPFNHHRNAGLRYRSMLLGNGRYVPFHIQVPQ
  KFFAVKNLLLLPGSYTYEWNFRKDVNMVLQSSLGNDLRVDGASISFTSINLYATFFPMAHNTASTLEAML
  RNDTNDQSFNDYLSAANMLYPIPANATNIPISIPSRNWAAFRGWSFTRLKTKETPSLGSGFDPYFVYSGS
  IPYLDGTFYLNHTFKKVSIMFDSSVSWPGNDRLLSPNEFEIKRTVDGEGYNVAQCNMTKDWFLVQMLANY
  NIGYQGFYIPEGYKDRMYSFFRNFQPMSRQVVDEVNYKDFKAVAIPYQHNNSGFVGYMAPTMRQGQPYPA
  NYPYPLIGTTAVNSVTQKKFLCDRTMWRIPFSSNFMSMGALTDLGQNMLYANSAHALDMTFEVDPMDEPT
  LLYLLFEVFDVVRVHQPHRGIIEAVYLRTPFSAGNATT
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011202
• ORF Size: 2844 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011202.1, YP_002213812
• RefSeq ORF: 2844
• Locus ID: 6870505
• MW: 107.0 kDa