E3 (NC_011202) Virus Tagged ORF Clone

CAT#: VC100318

Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein E3 CR1-beta [Human adenovirus B], codon optimized for human cell expression, YP_002213822

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100318 represents NCBI reference of YP_002213822 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCCCTCACAAGCCTGATTTTCGTTAGCATTGTGACTGCTGCCCATGGGCAAACCGTAGTTAGTA
  TTCCCCTGGGGCACAATTACACACTGATCGGGCCACCCATTACCAGTGAGGTCATCTGGACTAAGTTGGG
  GAGCGTAGACTACTTCGATATCATATGCAACAAGACCAAGCCCATCATTGTCACATGCAACATCCAGAAT
  CTCACACTGATTAACGTAAGCAAAGTTTACTCCGGGTACTATTATGGGTATGACAGATATTCCAGCCAGT
  ACCGAAATTACCTGGTAAGAGTGACACAATTGAAAACAACGAAAATGCCAAATATGGCCAAAATCAGAAG
  CGACGATAACAGTCTGGAAACCTTTACTAGCCCTACAACTCCCGACGAGAAGAACATTCCCGACTCTATG
  ATTGCGATCGTTGCAGCTGTGGCTGTGGTCATGGCATTGATCATAATATGTATGCTTCTGTACGCCTGCC
  GCTACAAGAAATTCCACCCCAAAAAGCAGGACTTGCTGCTGCGACTGAACATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100318 representing YP_002213822
  Red=Cloning sites Green=Tags
  
  MASLTSLIFVSIVTAAHGQTVVSIPLGHNYTLIGPPITSEVIWTKLGSVDYFDIICNKTKPIIVTCNIQN
  LTLINVSKVYSGYYYGYDRYSSQYRNYLVRVTQLKTTKMPNMAKIRSDDNSLETFTSPTTPDEKNIPDSM
  IAIVAAVAVVMALIIICMLLYACRYKKFHPKKQDLLLRLNI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011202
• ORF Size: 543 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011202.1, YP_002213822
• RefSeq ORF: 543
• Locus ID: 6870511
• MW: 20.4 kDa