E4 (NC_011202) Virus Tagged ORF Clone

CAT#: VC100323

Myc-DDK-tagged ORF clone of viral ORF for control protein E4 34K [Human adenovirus B], codon optimized for human cell expression, YP_002213847

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100323 represents NCBI reference of YP_002213847 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGGATCCAACTCCATCATGACCAGGCTGCGCGCTAGGAGCACGTCATGTGCCCGGCACCATCCTT
  ACACAAGGGCACAGCTCCCAAGGTGCGAGGAGAATGAAACAAGGGCTAGTATGACAGAAGATCACCCTCT
  GCTGCCTGATTGCGATACCATGACCATGCATAGTGTGTCTTGCGTCAGGGGTCTGCCGTGTTCTGCCAGC
  TTTACAGTGCTGCAAGAGCTGCCAATACCATGGGATATGTTTCTGAATCCGGAGGAACTCAAAATTATGC
  GACGCTGCATGCACCTTTGTCTCTGTTGCGCAACTATCGACATCTTTCACAGCCAGGTAATCCACGGCCG
  GGAGAATTGGGTTCTGCACTGTCATTGCAACCAACAGGGCAGTCTGCAATGCATGGCTGGCGGGGCCGTG
  TTGGCCGTGTGGTTCAGAAAGGTGATCCTGGGATGCATGATTAATCAGCGCTGCCCTTGGTATAGACAGA
  TTGTGAACATGCATATGCCTAAGGAGATAATGTACGTGGGGTCAGTTTTCTTGCGCGAGCGGCACTTGAT
  TTACATCAAGTTGTGGTACGACGGACACGCTGGAGCAATCATCTCTGATATGTCCTTTGGCTGGAGTGCC
  TTTAATTACGGGTTGCTCAACAATATCGTCATCATGTGCTGTACTTACTGTAAAGATTTGAGCGAGATCC
  GGATGAGATGCTGCGCCCACAGAACCAGGAAATTGATGCTCAGAGCCATCAAAATTATGCTGCAAGACAC
  CGTTGATCCCGATCCTATTAATAGCTCCAGAACTGAGCGGAGACGCCAGCGATTGCTGGTGGGACTCATG
  CGCCATAACCGACCCATACCCTTCTCTGACTACGATTCACACAGGTCTAGTAGTCGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100323 representing YP_002213847
  Red=Cloning sites Green=Tags
  
  MSGSNSIMTRLRARSTSCARHHPYTRAQLPRCEENETRASMTEDHPLLPDCDTMTMHSVSCVRGLPCSAS
  FTVLQELPIPWDMFLNPEELKIMRRCMHLCLCCATIDIFHSQVIHGRENWVLHCHCNQQGSLQCMAGGAV
  LAVWFRKVILGCMINQRCPWYRQIVNMHMPKEIMYVGSVFLRERHLIYIKLWYDGHAGAIISDMSFGWSA
  FNYGLLNNIVIMCCTYCKDLSEIRMRCCAHRTRKLMLRAIKIMLQDTVDPDPINSSRTERRRQRLLVGLM
  RHNRPIPFSDYDSHRSSSR
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011202
• ORF Size: 897 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011202.1, YP_002213847
• RefSeq ORF: 897
• Locus ID: 6870513
• MW: 34.7 kDa