L4 (NC_011202) Virus Tagged ORF Clone

CAT#: VC100300

Myc-DDK-tagged ORF clone of viral ORF for protein 33K [Human adenovirus B], codon optimized for human cell expression, YP_002213816

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100300 represents NCBI reference of YP_002213816 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCACAACGCCGAGAAAACAAGAAACTCAAAGTTCAGCCCCCCCCTCCAGAAGACATGGAAGAAGACT
  GGGACAGCCAGGCCGAGGAAGCTGAAGAAGATTCTCTTGAAGATTCCCTCGAGGAGGATTCACTCGAAGA
  AGAGAACGAAGAGGCGGAGGAGGTGGAGGAAGTCACAGCCGATAAGCAGCTCTCAAGCGCTGCGGAGACC
  TCTAACAGCGCTACAATTTCAGCCCCTTCTAGGGGGACCCGGCGACGCCCTTCCAGTAGATGGGACGAAA
  CCGGAAGATTCCCAAATCCGACCTCAGCGTCAAAGACAGCCCCTACCATTGCTAGCAAGAGCCGGCAGTC
  ACGGCAGATCAAGACGGCTGCCGCCACGTCTAATAGAAAGCCAGCCGCGGCCGTTCGGAAGTACACCACA
  TCCGCCGCCACAGGTGGTCTTAAGATTACCGCAAACGAGCCAGCCCAAACAAGAGAGCTGAGGAATCGCA
  TATTCCCAACCCTGTATGCCATCTTCCAGCAATCCCGAGGTCAGGAACAGGAACTGAAGATCAAGAACAG
  ATCCCTGAGATCTCTTACCAGATCCTGTCTTTATCACAAATCAGAAGACCAGCTCCAGCGGACGCTCGAA
  GACGCCGAAGCCTTGTTCAACAAGTATTGCGCCCTGACGCTGAAGGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100300 representing YP_002213816
  Red=Cloning sites Green=Tags
  
  MSQRRENKKLKVQPPPPEDMEEDWDSQAEEAEEDSLEDSLEEDSLEEENEEAEEVEEVTADKQLSSAAET
  SNSATISAPSRGTRRRPSSRWDETGRFPNPTSASKTAPTIASKSRQSRQIKTAAATSNRKPAAAVRKYTT
  SAATGGLKITANEPAQTRELRNRIFPTLYAIFQQSRGQEQELKIKNRSLRSLTRSCLYHKSEDQLQRTLE
  DAEALFNKYCALTLKE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011202
• ORF Size: 678 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011202.1, YP_002213816
• RefSeq ORF: 678
• Locus ID: 6870504
• MW: 25.4 kDa