L2 (NC_011202) Virus Tagged ORF Clone

CAT#: VC100305

Myc-DDK-tagged ORF clone of viral ORF for core protein precursor pX [Human adenovirus B], codon optimized for human cell expression, YP_002213810

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100305 represents NCBI reference of YP_002213810 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTCACATGTAGACTCCGGGTGCCTATCACCGGGTATCGCGGCCGAAATTCTCGGAGGAGGAGAG
  GAATGCTTGGACGAGGCATGCGCCGATACCGGCGGCGGAGAGCTATCCGCAAGCAGCTCAGAGGTGGGTT
  TCTGCCTGCGCTCATACCCATTATCGCGGCTGCTATTGGGGCCATCCCAGGAATCGCTTCTGTGGCAGTC
  CAGGCTAGTCAGAGGCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100305 representing YP_002213810
  Red=Cloning sites Green=Tags
  
  MALTCRLRVPITGYRGRNSRRRRGMLGRGMRRYRRRRAIRKQLRGGFLPALIPIIAAAIGAIPGIASVAV
  QASQRH
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011202
• ORF Size: 228 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011202.1, YP_002213810
• RefSeq ORF: 228
• Locus ID: 6870506
• MW: 8.5 kDa