L5 (NC_011202) Virus Tagged ORF Clone

CAT#: VC100297

Myc-DDK-tagged ORF clone of viral ORF for fiber [Human adenovirus B], codon optimized for human cell expression, YP_002213828

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100297 represents NCBI reference of YP_002213828 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTAAGAGGGTTAGACTGAGTGATAGTTTCAATCCTGTTTACCCGTATGAAGATGAGAGCACGAGTC
  AACACCCGTTTATCAACCCCGGGTTCATTTCCCCAAATGGTTTTACACAGTCTCCTAATGGTGTCCTCAC
  CCTGAAGTGTCTTACTCCCCTGACCACCACCGGCGGAAGCTTGCAGCTGAAAGTGGGTGGAGGGCTTACC
  GTCGATGACACTAATGGCTTCCTCAAAGAGAACATATCCGCCACCACACCGCTTGTGAAGACTGGGCATA
  GCATCGGACTCCCCCTGGGAGCGGGGCTGGGCACGAACGAGAATAAACTCTGCATCAAACTGGGACAGGG
  CCTGACTTTTAACAGCAACAATATCTGTATCGATGATAATATTAATACGCTTTGGACTGGCGTGAACCCT
  ACAGAAGCCAACTGTCAAATTATGAACTCCAGCGAGTCTAACGACTGTAAACTGATACTTACACTGGTCA
  AGACCGGCGCTCTGGTGACTGCCTTTGTGTATGTGATTGGCGTTAGCAACAATTTTAACATGCTCACCAC
  ACACCGGAATATTAATTTCACTGCTGAACTGTTTTTCGATTCTACTGGGAATTTGCTGACGAGACTGAGC
  AGTCTCAAGACACCCCTGAACCACAAGAGCGGCCAGAATATGGCCACAGGGGCAATCACAAATGCGAAGG
  GATTTATGCCTTCTACAACCGCCTATCCATTTAACGATAACTCTCGAGAAAAAGAGAACTATATCTATGG
  TACATGTTACTACACAGCAAGCGATAGGACCGCCTTCCCCATTGACATCTCAGTTATGCTGAACAGGCGC
  GCCATCAACGATGAGACATCATATTGTATAAGGATAACCTGGAGCTGGAATACAGGCGACGCACCCGAAG
  TTCAGACCTCTGCCACGACCTTGGTAACATCACCTTTCACCTTTTACTATATCAGGGAGGACGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100297 representing YP_002213828
  Red=Cloning sites Green=Tags
  
  MTKRVRLSDSFNPVYPYEDESTSQHPFINPGFISPNGFTQSPNGVLTLKCLTPLTTTGGSLQLKVGGGLT
  VDDTNGFLKENISATTPLVKTGHSIGLPLGAGLGTNENKLCIKLGQGLTFNSNNICIDDNINTLWTGVNP
  TEANCQIMNSSESNDCKLILTLVKTGALVTAFVYVIGVSNNFNMLTTHRNINFTAELFFDSTGNLLTRLS
  SLKTPLNHKSGQNMATGAITNAKGFMPSTTAYPFNDNSREKENYIYGTCYYTASDRTAFPIDISVMLNRR
  AINDETSYCIRITWSWNTGDAPEVQTSATTLVTSPFTFYYIREDD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011202
• ORF Size: 975 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011202.1, YP_002213828
• RefSeq ORF: 975
• Locus ID: 6870503
• MW: 35.5 kDa