L2 (NC_011202) Virus Tagged ORF Clone

CAT#: VC100308

Myc-DDK-tagged ORF clone of viral ORF for penton base [Human adenovirus B], codon optimized for human cell expression, YP_002213807

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100308 represents NCBI reference of YP_002213807 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCGCGTGGTGCTGGGAGGCGCTGTTGTGTACCCAGAGGGTCCACCCCCATCTTATGAGTCTGTTA
  TGCAGCAACAGCAAGCGACGGCCGTGATGCAGTCCCCACTGGAGGCACCCTTCGTGCCCCCCCGATACCT
  GGCTCCAACGGAGGGGAGAAACTCAATTAGATACTCTGAGCTGGCACCCCAGTACGACACCACTAGACTC
  TATCTCGTGGACAACAAAAGTGCTGATATTGCATCCCTTAACTATCAGAACGACCATTCTAATTTCCTGA
  CAACGGTGGTCCAGAATAATGATTTCACTCCAACCGAAGCCTCAACTCAAACAATCAACTTCGACGAGAG
  GAGCCGCTGGGGTGGGCAGTTGAAAACTATTATGCATACGAATATGCCCAATGTCAACGAATATATGTTT
  AGCAACAAGTTTAAGGCTCGCGTCATGGTTAGTCGGAAGCCGCCCGATGGCGCGGCAGTCGGCGATACTT
  ACGACCATAAACAGGACATTCTGAAGTACGAATGGTTCGAATTTACACTTCCTGAGGGTAATTTTAGTGT
  GACTATGACCATCGATCTGATGAATAACGCCATTATTGATAATTATCTGAAAGTGGGCAGACAGAACGGC
  GTCTTGGAGAGCGATATTGGGGTGAAATTCGACACACGGAACTTCAAATTGGGATGGGACCCAGAGACTA
  AGTTGATTATGCCCGGTGTCTATACTTACGAAGCGTTCCACCCAGATATCGTGTTGCTCCCTGGCTGCGG
  CGTGGACTTCACCGAGAGCCGCCTCAGTAACCTGTTGGGAATTCGGAAAAAGCAGCCCTTCCAGGAAGGC
  TTCAAAATTCTCTACGAAGACCTCGAAGGAGGCAATATTCCTGCACTGCTCGACGTCGACGCTTATGAGA
  ATTCAAAGAAGGAGCAGAAAGCAAAAATCGAGGCAGCCACGGCCGCAGCCGAGGCGAAAGCAAACATCGT
  GGCCTCCGACTCAACGAGAGTTGCCAATGCCGGAGAAGTCAGAGGGGACAATTTTGCCCCTACACCCGTG
  CCAACAGCAGAATCCCTCCTCGCTGATGTGTCCGAAGGCACAGATGTGAAACTTACCATCCAACCAGTCG
  AGAAGGACTCTAAGAATCGGTCATACAACGTCCTTGAAGATAAGATCAATACCGCGTATAGAAGCTGGTA
  CCTCAGTTACAACTACGGCGATCCCGAGAAGGGTGTACGGAGTTGGACACTGCTCACGACGAGCGACGTC
  ACGTGCGGGGCTGAACAAGTATACTGGAGCCTGCCAGACATGATGAAGGACCCTGTGACATTTCGCAGTA
  CTCGCCAGGTTAGTAACTATCCCGTGGTGGGAGCAGAGCTCATGCCAGTGTTTTCAAAGTCTTTTTACAA
  CGAGCAAGCAGTTTACTCCCAGCAACTGCGACAATCAACCTCCCTGACCCATGTGTTCAATCGGTTCCCA
  GAGAATCAGATCCTCATTCGGCCTCCAGCCCCCACTATTACCACGGTGAGTGAGAATGTGCCTGCGCTGA
  CCGACCACGGCACTCTGCCTCTGCGCAGCAGCATCAGGGGCGTGCAGCGCGTAACAGTAACTGATGCACG
  GAGGCGGACATGCCCGTACGTGTACAAGGCCCTGGGTATCGTGGCGCCTAGAGTGCTCAGCTCAAGGACT
  TTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100308 representing YP_002213807
  Red=Cloning sites Green=Tags
  
  MRRVVLGGAVVYPEGPPPSYESVMQQQQATAVMQSPLEAPFVPPRYLAPTEGRNSIRYSELAPQYDTTRL
  YLVDNKSADIASLNYQNDHSNFLTTVVQNNDFTPTEASTQTINFDERSRWGGQLKTIMHTNMPNVNEYMF
  SNKFKARVMVSRKPPDGAAVGDTYDHKQDILKYEWFEFTLPEGNFSVTMTIDLMNNAIIDNYLKVGRQNG
  VLESDIGVKFDTRNFKLGWDPETKLIMPGVYTYEAFHPDIVLLPGCGVDFTESRLSNLLGIRKKQPFQEG
  FKILYEDLEGGNIPALLDVDAYENSKKEQKAKIEAATAAAEAKANIVASDSTRVANAGEVRGDNFAPTPV
  PTAESLLADVSEGTDVKLTIQPVEKDSKNRSYNVLEDKINTAYRSWYLSYNYGDPEKGVRSWTLLTTSDV
  TCGAEQVYWSLPDMMKDPVTFRSTRQVSNYPVVGAELMPVFSKSFYNEQAVYSQQLRQSTSLTHVFNRFP
  ENQILIRPPAPTITTVSENVPALTDHGTLPLRSSIRGVQRVTVTDARRRTCPYVYKALGIVAPRVLSSRT
  F
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011202
• ORF Size: 1683 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011202.1, YP_002213807
• RefSeq ORF: 1683
• Locus ID: 6870506
• MW: 62.9 kDa