E4 (NC_011202) Virus Tagged ORF Clone

CAT#: VC100326

Myc-DDK-tagged ORF clone of viral ORF for control protein E4orf2 [Human adenovirus B], codon optimized for human cell expression, YP_002213850

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100326 represents NCBI reference of YP_002213850 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGAGCGCAGTGCCGTTACCTACTGCATCTGTATCCCCGAAGTGCTGAACGTGTTTCTCGCCAACT
  TCAGTTTCATACAGTTCTTGCAAGAGACGTTGCCCTATTACATCAGTAGCAATTTTGACGATATCACAGG
  CGGATCCCAGTATGCTTACAGCAACTTGGTGTTCGCAGGAGCTAACTGGGGCGGTCTGCGGTTGTATTGC
  ACAGTTGCAAGCCCTGCTCTGGTTCCTGGAGGCTTGCTCGCTAAGCAGTTTGGTGACAATATGAGGGAAT
  TCCTTCAGCTCGAGCTGAAAGAAGAGCTCAGAGCCAAAGGAATGTCAATAGACGTGGCCATTCTGAACTC
  ACTTCAGGTTACCCAGGAACAAGACATCCTGTCCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100326 representing YP_002213850
  Red=Cloning sites Green=Tags
  
  MLERSAVTYCICIPEVLNVFLANFSFIQFLQETLPYYISSNFDDITGGSQYAYSNLVFAGANWGGLRLYC
  TVASPALVPGGLLAKQFGDNMREFLQLELKEELRAKGMSIDVAILNSLQVTQEQDILSL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011202
• ORF Size: 387 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011202.1, YP_002213850
• RefSeq ORF: 387
• Locus ID: 6870513
• MW: 14.3 kDa