E2B (NC_011202) Virus Tagged ORF Clone

CAT#: VC100295

Myc-DDK-tagged ORF clone of viral ORF for terminal protein precursor pTP [Human adenovirus B], codon optimized for human cell expression, YP_002213843

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100295 represents NCBI reference of YP_002213843 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTGAGTATTAACGATTGTGCACGCCTGACCGGTCAGACTGTTCCGACCATGAATTACTTTATGC
  CACTGCGCAACATTTGGAATCGGGTGCGCGAATTCCCCCGCGCCTCTACCACAGCAGCCGGGATCACTTG
  GATGAGCCGGTACATTTATGGGTACCACCGGTTGATGCTCGAAGACCTGGCGCCTGGCGCACCCGCTACG
  GAGCGCTGGCCTCTTTACCGCCAGCCTCCTCCACACTTCCTTATTGGCTACCAGTACCTGGTCAGGACCT
  GTAATGATTACATATTTGATACACGAGCTTATAGCAGACTGAAGTATACTGAGCTGGTGCGGCCCGGGCA
  TCAGACTGTGAACTGGTCTGTCATGGCCAACTGTGCCTATACTATCAACACCGGTGCGTACCACCGGTTT
  GTCGATTTCGACGACTTCCAGGCGACTCTGACCCAGGTCCAGCAAGCCATTCTTGCAGAGCGCGTAGTTG
  CTGACCTCGCGCTGGTTCAACCGATGCGAGGGTTCGGTCTTACACGGATGCACGGGAGGACAGGCGAGGA
  AGAGGTCCCCGTGGAGCGGTTGATGCAAGACTACTACAAGGACCTCGCACGGTGCCAGGACAACGCTTGG
  GGTATGGCCAACCGACTTCGGATCCAGCAAGCTGGCCCAAAAGACCTCGTTCTTCTCGCCACCATCCGGC
  GGCTCCGAACGGCGTATTTCAACTTCATCACGTCTAGCATAGTGTCCCCATCCCGGGACGAGGAGGAGAC
  CGAGGACCCAGACCGAACTCACAACCACCCTCGACCGCAGGAGGCAGTTCTGAGTCTTCCATGCGACTGT
  GACTGGCTCGATGCATTCGTTGAACGGTTCAGCGACCCAGTCGACCTGGATACAATCAGGTCTTTGAGAG
  CCGTACCTACAGGCCAGCTCATAAAATGTATTATCAGCGCACTGAGCCTGCCCAACGGCGAGCCTCCCTC
  CCATCATTTTAAGGAAATGCGAGGAGGCGTCTTTACATTGCGGCCAAGGGAAAACGGAAGGGCCGTGACT
  GAGACCATGCGAAGGCGCAGAGGCGAAGTTATCGAGCGCTTCATCGACCGCCTGCCCGTTCGGCGACGAC
  GAAGACGGGTGCCACCTCCTCCCCCACCTGAAGACGAAGAGGAGATGTTGGTCGAGGAAGAGATAGAGGA
  GGAAGAGGAAATTCCCGGTGCTTTCGAGCGGGAAGTTCGGGCCACAATAGCAGAACTCATTCGGTTGCTG
  GAGGAAGAGCTGACTGTGTCCGCAAGGAATTCACAGTTCTTTAATTTTGCGGTGGACTTCTACGAGGCGA
  TGGAAAGGCTTGAGGCGCTTGGGGACGTCTCCGAAATGCCTCTGCGGCGGTGGATCATGTATTTCTTCGT
  GGCCGAACACATCGCGACCACCCTTAACTACCTCTTCCAAAGGCTTTGCAACTATGCCGTCTTCACCCGC
  CACGTGGAACTTAACCTCGCTCAGGTCGTCATGCGCGCCCGGGACAGCGAAGGTGCTGTAGTCTACAGCC
  GCGTTTGGAATGAAGCTGGTATGAACGCATTCTCACAACTTATGGGTAGGATTTCCAACGACCTTGCGGC
  CACGGTCGAACGGGCAGGCCGAGGGGACCTCCAGGAGGAAGAGATCGAGCAGTTTATGGCCGAAATAGCT
  TACCAAGATAATAGCGGGGACGTTCAGGAGATACTTAGGCAGGCCGCCGTTAACGATACTGAGATTGATT
  CAGTGGAACTCTCTTTCAGGTTTAAGCTTACGGGTCCAGTTGCATTCACCCAGCGGCGCCAGATTCAGGA
  CGTCAATCGACGGGTTGTGGCACATGCTAGCCTGCTGCGCGCACAATACCAGAATTTGCCCGTCCGCGGA
  GCTGATGTGCCCTTGCCAGCCATGCCAAGCGGACCTGAACAGCCCCTGCCTCCTGGCGCAAGACCCAGGC
  ACAGGTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100295 representing YP_002213843
  Red=Cloning sites Green=Tags
  
  MALSINDCARLTGQTVPTMNYFMPLRNIWNRVREFPRASTTAAGITWMSRYIYGYHRLMLEDLAPGAPAT
  ERWPLYRQPPPHFLIGYQYLVRTCNDYIFDTRAYSRLKYTELVRPGHQTVNWSVMANCAYTINTGAYHRF
  VDFDDFQATLTQVQQAILAERVVADLALVQPMRGFGLTRMHGRTGEEEVPVERLMQDYYKDLARCQDNAW
  GMANRLRIQQAGPKDLVLLATIRRLRTAYFNFITSSIVSPSRDEEETEDPDRTHNHPRPQEAVLSLPCDC
  DWLDAFVERFSDPVDLDTIRSLRAVPTGQLIKCIISALSLPNGEPPSHHFKEMRGGVFTLRPRENGRAVT
  ETMRRRRGEVIERFIDRLPVRRRRRRVPPPPPPEDEEEMLVEEEIEEEEEIPGAFEREVRATIAELIRLL
  EEELTVSARNSQFFNFAVDFYEAMERLEALGDVSEMPLRRWIMYFFVAEHIATTLNYLFQRLCNYAVFTR
  HVELNLAQVVMRARDSEGAVVYSRVWNEAGMNAFSQLMGRISNDLAATVERAGRGDLQEEEIEQFMAEIA
  YQDNSGDVQEILRQAAVNDTEIDSVELSFRFKLTGPVAFTQRRQIQDVNRRVVAHASLLRAQYQNLPVRG
  ADVPLPAMPSGPEQPLPPGARPRHRF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011202
• ORF Size: 1968 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011202.1, YP_002213843
• RefSeq ORF: 1968
• Locus ID: 6870501
• MW: 75.6 kDa