L4 (NC_011202) Virus Tagged ORF Clone

CAT#: VC100299

Myc-DDK-tagged ORF clone of viral ORF for encapsidation protein 22K [Human adenovirus B], codon optimized for human cell expression, YP_002213817

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100299 represents NCBI reference of YP_002213817 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCACAGCGAAGAGAGAATAAGAAACTGAAAGTGCAGCCCCCTCCACCAGAGGACATGGAGGAGGATT
  GGGACTCCCAGGCCGAGGAGGCGGAAGAAGATAGTCTCGAGGATTCCCTGGAAGAAGACTCCCTCGAAGA
  AGAAAATGAAGAAGCTGAAGAGGTCGAGGAGGTGACAGCCGACAAACAGCTCTCATCTGCAGCTGAGACA
  TCAAATTCTGCAACAATAAGTGCTCCAAGCAGGGGAACTAGGCGGCGGCCGTCCTCTCGGTGGGACGAAA
  CCGGACGCTTCCCTAACCCCACATCCGCCTCCAAAACCGGAAAAAAAGACCGACAGGGGTACAAGTCCTG
  GCGAGGGCACAAGAATGCCATCATCTCATGCCTGCACGAGTGCGGTGGGAACATCTCTTTTACTCGCAGA
  TACCTGCTGTTCCACCATGGAGTGAACTTCCCTCGGAACGTGCTGCACTATTACCGCCATCTTCACAGCC
  CCTACTATTCTCAACAAATTCCTGCTGTGAGTACTGACAAGGATAGCGGTGGCGATCTTCAACAGAAGAC
  TTCTAGCGGCTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100299 representing YP_002213817
  Red=Cloning sites Green=Tags
  
  MSQRRENKKLKVQPPPPEDMEEDWDSQAEEAEEDSLEDSLEEDSLEEENEEAEEVEEVTADKQLSSAAET
  SNSATISAPSRGTRRRPSSRWDETGRFPNPTSASKTGKKDRQGYKSWRGHKNAIISCLHECGGNISFTRR
  YLLFHHGVNFPRNVLHYYRHLHSPYYSQQIPAVSTDKDSGGDLQQKTSSGS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011202
• ORF Size: 573 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011202.1, YP_002213817
• RefSeq ORF: 573
• Locus ID: 6870504
• MW: 21.7 kDa