E1B (NC_011202) Virus Tagged ORF Clone

CAT#: VC100292

Myc-DDK-tagged ORF clone of viral ORF for control protein E1B 55K [Human adenovirus B], codon optimized for human cell expression, YP_002213800

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100292 represents NCBI reference of YP_002213800 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCCAGCAGATTCTTTTCAGCAGGGGATTCGGTTTGGATTCCACTCACACAGTATAGTCGAGAATA
  TGGAGGGATCCCAGGATGAGGACAATTTGCGACTGCTGGCAAGTGCAGCATTTGGCTGTAGTGGTAACCC
  AGAAGCTAGTACTGGACATGCTAGTGGCTCAGGCGGAGGAACAGCCAGAGGACAGCCTGAAAGTCGGCCT
  GGACCTAGCTCCGGGGGGGGAGGGGTAGCTGACCTGAGCCCCGAGCTGCAGAGAGTGCTTACCGGCTCAA
  CCAGCACCGGTAGAGATCGGGGTGTGAAGCGCGAGAGAGCTTCCTCCGGGACTGACGCCCGATCAGAACT
  CGCACTGTCTCTGATGTCCCGCAGGAGACCGGAAACGATCTGGTGGCACGAGGTACAGAAGGAGGGACGC
  GACGAGGTGTCTGTCCTCCAAGAGAAATATTCACTCGAGCAGGTGAAGACCTGCTGGCTGGAGCCCGAAG
  ATGATTGGGAAGTTGCTATCAAGAATTACGCAAAAATTGCCCTGCGCCCAGACAAACAATACAAAATCAC
  CAGGCGGATAAACATTCGCAACGCCTGTTACATTTCAGGTAATGGCGCCGAAGTGGTGATCGATACTCAG
  GACAAAGCCGTGATTAGGTGCTGCATGATGGACATGTGGCCAGGGGTGGTTGGCATGGAAGCAGTCACAT
  TTGTTAACGTTAAATTTCGCGGGGACGGATATAATGGAATCGTGTTTATGGCAAACACAAAACTCATTCT
  CCATGGTTGCAGTTTTTTTGGGTTTAACAACACGTGTGTTGATGCCTGGGGCCAAGTGTCCGTACGGGGC
  TGTTCCTTCTACGCATGCTGGATTGCGACCGCCGGGAGAACCAAATCACAGCTCTCCCTCAAGAAATGTA
  TCTTCCAGCGCTGCAATCTCGGCATCTTGAACGAGGGGGAGGCCCGCGTTCGCCACTGCGCCTCTACAGA
  CACGGGTTGCTTCATTCTTATCAAGGGCAATGCCAGTGTTAAACACAATATGATATGCGGGGCCAGCGAT
  GAACGCCCCTACCAGATGCTCACATGTGCAGGAGGCCATTGCAATATGCTTGCCACCGTGCACATAGTGT
  CTCATCAGAGAAAAAAGTGGCCCGTGTTCGACCACAACGTGATGACTAAGTGCACTATGCACGCTGGCGG
  GCGGAGAGGTATGTTCATGCCGTACCAGTGTAATATGAATCATGTAAAGGTGCTGCTGGAACCCGATGCT
  TTCAGCAGAATGTCACTCACAGGCATCTTCGATATGAATATGCAAATATGGAAGATCTTGCGCTATGACG
  ACACCCGCTCTCGCGTGAGGGCATGCGAATGCGGTGGGAAGCATGCCCGCTTTCAACCGGTTTGCGTGGA
  CGTCACAGAGGACTTGCGACCCGATCACCTGGTAATCGCTCGGACAGGCGCAGAGTTCGGATCCTCTGGG
  GAGGAGACTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100292 representing YP_002213800
  Red=Cloning sites Green=Tags
  
  MDPADSFQQGIRFGFHSHSIVENMEGSQDEDNLRLLASAAFGCSGNPEASTGHASGSGGGTARGQPESRP
  GPSSGGGGVADLSPELQRVLTGSTSTGRDRGVKRERASSGTDARSELALSLMSRRRPETIWWHEVQKEGR
  DEVSVLQEKYSLEQVKTCWLEPEDDWEVAIKNYAKIALRPDKQYKITRRINIRNACYISGNGAEVVIDTQ
  DKAVIRCCMMDMWPGVVGMEAVTFVNVKFRGDGYNGIVFMANTKLILHGCSFFGFNNTCVDAWGQVSVRG
  CSFYACWIATAGRTKSQLSLKKCIFQRCNLGILNEGEARVRHCASTDTGCFILIKGNASVKHNMICGASD
  ERPYQMLTCAGGHCNMLATVHIVSHQRKKWPVFDHNVMTKCTMHAGGRRGMFMPYQCNMNHVKVLLEPDA
  FSRMSLTGIFDMNMQIWKILRYDDTRSRVRACECGGKHARFQPVCVDVTEDLRPDHLVIARTGAEFGSSG
  EETD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011202
• ORF Size: 1482 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011202.1, YP_002213800
• RefSeq ORF: 1482
• Locus ID: 6870499
• MW: 54.6 kDa