L4 (NC_011202) Virus Tagged ORF Clone

CAT#: VC100301

Myc-DDK-tagged ORF clone of viral ORF for hexon assembly protein 100K [Human adenovirus B], codon optimized for human cell expression, YP_002213815

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100301 represents NCBI reference of YP_002213815 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACTCAGCCTCTCCTGTCTACATCACCAAGGGTGCCCAGTCACCTTGTCTTGTCAGATGAAGAGA
  AGGAGCAGAGTTTGTCAATTCCCCCTCCTAGCCCGGCGACCACCAGCACCCTTGAGGACAAGGAGGTGGA
  TGCTAGTCATGACATGCAGAACAAGAAAGCCAAGGAATCAGAAACTGATATTGAACAGGACCCTGGCTAC
  GTGACACCCGTAGAACACGAAGAGGAGCTGAAAAGGTTTTTGGAGAGAGAGGACGAGAATTGCCCTAAGC
  AGAGGGCTGACAACTACCACCAGGATGCAGGGAATCGGGACCAGAATACCGACTATCTGATCGGACTGGA
  CGGGGAAGATGCTCTGCTGAAGCATCTGGCCAGACAATCCCTGATCGTTAAAGACGCCCTGCTTGATAGA
  ACCGAAGTGCCAATCAGTGTGGAAGAGTTGTCCCGCGCCTATGAGCTGAACCTGTTTTCTCCCAGGACTC
  CCCCCAAAAGACAGCCAAACGGGACGTGTGAACCGAATCCCAGACTTAATTTCTATCCCGCTTTCGCCGT
  TCCAGAGGTATTGGCTACTTATCACATCTTCTTTAAGAATCAGAAGATCCCAGTAAGTTGTCGGGCCAAC
  CGAACAAGGGCAGACGCCTTGTTGAATCTTGGCCCCGGTAGCAGACTGCCAGACATTGCTTCTCTGGAGG
  AAGTGCCCAAGATCTTTGAAGGCCTGGGCAACAATGAAACACGCGCTGCAAATGCCCTTCAGAAAGGCGA
  GAATGGCATGGATGAGCACCACAGCGTGCTGGTGGAGCTGGAAGGTGACAACGCTCGGCTGGCGGTCCTG
  AAAAGGTCTATCGAAGTCACGCATTTCGCCTATCCTGCTGTCAATCTGCCGCCAAAAGTAATGACTGCCG
  TCATGGATCAGTTGCTCATCAAGCGAGCCAGCCCCCTGAGTGAGGACATGCACGACCCAGATGCCTGTGA
  TGAGGGGAAACCTGTGGTAAGTGATGAGCAACTGACTCGCTGGCTGGGAACGGACAGCCCGCGGGACTTG
  GAGGAAAGGCGGAAATTGATGATGGCCGTCGTGCTGGTCACCGTGGAGCTCGAGTGCCTGAGGAGGTTTT
  TCACTGACAGTGAAACGCTGAGGAAACTGGAAGAGAACCTGCACTATACTTTCCGACACGGTTTCGTGAG
  ACAGGCTTGCAAAATATCTAACGTAGAGCTTACTAACTTGGTGTCATACATGGGTATCCTCCACGAAAAC
  AGATTGGGACAGAGTGTTCTGCACTCCACACTTAAAGGCGAAGCAAGGAGGGACTATATCCGCGATTGCG
  TCTATTTGTACCTTTGTCATACCTGGCAAACAGGCATGGGCGTATGGCAACAGTGCCTGGAAGAACAGAA
  CTTGAAGGAGCTGGATAAGTTGCTTCAGAAAAGCTTGAAGGTCTTGTGGACCGGATTCGATGAACGAACT
  GTTGCTAGCGATCTGGCCGACCTGATATTCCCCGAGAGATTGCGGGTTACCCTGCGCAATGGCCTGCCCG
  ATTTTATGTCCCAGTCAATGCTGAACAATTTTAGGAGTTTCATTCTGGAACGCTCCGGCATACTGCCCGC
  TACCTGCTGTGCCCTGCCCAGCGATTTTGTGCCCCTCACATATCGAGAGTGCCCCCCTCCCTTGTGGAGC
  CACTGCTATCTGTTTCGACTCGCAAACTATCTCTCCTATCATTCTGATGTGATCGAGGATGTGTCCGGCG
  ACGGCCTCCTTGAGTGCCATTGCAGATGTAATCTTTGCACCCCACATAGGTCCCTCGCGTGTAACCCCCA
  GCTCATGAGCGAAACTCAGATTATTGGCACTTTCGAGCTGCAGGGGCCTAGCTCCCAGGGTGACGGCTCC
  AGCCCAGGACAGTCACTGAAACTGACCCCCGGCCTGTGGACAAGCGCCTACCTTAGGAAATTCGCTCCTG
  AAGATTACCACCCATATGAGATTAAATTTTACGAGGACCAGAGTCAGCCTCCCAAAGCCGAACTGTCTGC
  TTGCGTGATCACGCAGGGCGCGATCCTTGCGCAGCTGCAGGCCATCCAGAAATCTCGGCAGGAGTTTCTT
  CTGAAAAAGGGAAAAGGGGTGTATCTCGATCCTCAGACCGGCGAAGAGCTGAATACCAGATTTCCACAAG
  ACGTGCCCACCACCAGAAAACAGGAGGTTGAGGGTGCAGCAGCCGCCCCTCGGAGGTACGGAGGCAGACT
  TGGGCAATCTGGTCGCGGAGGAGGCGGCGGCCAGTCTGGGGGTCAATCTGGAGGCAGACAATTCGGTGGA
  GGCAAACGCGGGGGCAGAGGAGGAGGCAGATCAAATAGACGCCAGACGGTCATCCTTGGGTGTGGCGACA
  AGCAGCAGCGATACCATCTCAGAAGCGAGTCAAGGAACCCCGCCGCTAGCCAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100301 representing YP_002213815
  Red=Cloning sites Green=Tags
  
  METQPLLSTSPRVPSHLVLSDEEKEQSLSIPPPSPATTSTLEDKEVDASHDMQNKKAKESETDIEQDPGY
  VTPVEHEEELKRFLEREDENCPKQRADNYHQDAGNRDQNTDYLIGLDGEDALLKHLARQSLIVKDALLDR
  TEVPISVEELSRAYELNLFSPRTPPKRQPNGTCEPNPRLNFYPAFAVPEVLATYHIFFKNQKIPVSCRAN
  RTRADALLNLGPGSRLPDIASLEEVPKIFEGLGNNETRAANALQKGENGMDEHHSVLVELEGDNARLAVL
  KRSIEVTHFAYPAVNLPPKVMTAVMDQLLIKRASPLSEDMHDPDACDEGKPVVSDEQLTRWLGTDSPRDL
  EERRKLMMAVVLVTVELECLRRFFTDSETLRKLEENLHYTFRHGFVRQACKISNVELTNLVSYMGILHEN
  RLGQSVLHSTLKGEARRDYIRDCVYLYLCHTWQTGMGVWQQCLEEQNLKELDKLLQKSLKVLWTGFDERT
  VASDLADLIFPERLRVTLRNGLPDFMSQSMLNNFRSFILERSGILPATCCALPSDFVPLTYRECPPPLWS
  HCYLFRLANYLSYHSDVIEDVSGDGLLECHCRCNLCTPHRSLACNPQLMSETQIIGTFELQGPSSQGDGS
  SPGQSLKLTPGLWTSAYLRKFAPEDYHPYEIKFYEDQSQPPKAELSACVITQGAILAQLQAIQKSRQEFL
  LKKGKGVYLDPQTGEELNTRFPQDVPTTRKQEVEGAAAAPRRYGGRLGQSGRGGGGGQSGGQSGGRQFGG
  GKRGGRGGGRSNRRQTVILGCGDKQQRYHLRSESRNPAASQQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011202
• ORF Size: 2436 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011202.1, YP_002213815
• RefSeq ORF: 2436
• Locus ID: 6870504
• MW: 91.1 kDa