E3 (NC_011202) Virus Tagged ORF Clone

CAT#: VC100313

Myc-DDK-tagged ORF clone of viral ORF for control protein E3 125K [Human adenovirus B], codon optimized for human cell expression, YP_002213819

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100313 represents NCBI reference of YP_002213819 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGGAGACGCAGCCGAGCTGTCACGACTGCGCCATCTTGACCACTGCCGGCGATTCCGATGTTTCG
  CCCGCGAACTGATAGAGTTCATTTATTTTGAGCTTCCGAAGGATCATCCACAGGGGCCGGCCCATGGCGT
  TAGAATTTCAATCGAGGGGAAGATCGACAGTAGACTCCAGAGAATCTTTTCACAGAGGCCTGTCTTGATC
  GAACGCGATCAAGGAAATACAACGGTGTCCATCTATTGCATCTGTAATCACCCCGGACTGCATGAAAGCC
  TTTGTTGCCTGATGTGTACTGAATTCAATAAGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100313 representing YP_002213819
  Red=Cloning sites Green=Tags
  
  MSGDAAELSRLRHLDHCRRFRCFARELIEFIYFELPKDHPQGPAHGVRISIEGKIDSRLQRIFSQRPVLI
  ERDQGNTTVSIYCICNHPGLHESLCCLMCTEFNKN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011202
• ORF Size: 315 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011202.1, YP_002213819
• RefSeq ORF: 315
• Locus ID: 6870511
• MW: 12.2 kDa