L3 (NC_011202) Virus Tagged ORF Clone

CAT#: VC100304

Myc-DDK-tagged ORF clone of viral ORF for capsid protein precursor pVI [Human adenovirus B], codon optimized for human cell expression, YP_002213811

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100304 represents NCBI reference of YP_002213811 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGACATTAACTTCAGTAGTCTCGCCCCCAGACATGGCACCAAACCTTACATGGGAACTTGGAGCG
  ATATCGGCACTTCACAGCTGAACGGAGGGGCATTTAACTGGTCCAGTATTTGGTCAGGACTGAAAAACTT
  TGGTTCTACTATCAAGACATATGGCAACAAGGCGTGGAACTCCAGCACCGGCCAGGCCCTGAGAAATAAA
  CTGAAGGACCAGAATTTTCAGCAGAAGGTAGTCGACGGTATCGCCAGCGGAATAAATGGAGTGGTGGACC
  TGGCAAATCAAGCCGTGCAAAAGAAAATTAACAGTAGGCTCGACCCACCACCAGCAACGCCCGGGGAAAT
  GCAAGTGGAAGAGGAAATTCCTCCTCCCGAAAAAAGGGGCGATAAGCGGCCTCGGCCAGACCTGGAGGAG
  ACGCTGGTAACACGGGTGGACGAACCCCCCAGCTACGAGGAGGCCACAAAACTTGGTATGCCTACCACCA
  GACCAATTGCCCCAATGGCTACAGGCGTAATGAAACCTAGTCAACTTCATCGCCCTGTTACCCTGGATTT
  GCCCCCTCCACCAGCCGCAACAGCCGTGCCGGCAAGTAAGCCCGTAGCCGCTCCCAAACCAGTCGCAGTC
  GCGCGATCACGCCCTGGAGGTGCTCCGAGACCAAACGCACACTGGCAGAATACTCTCAACTCTATCGTGG
  GCCTGGGAGTACAGTCCGTTAAACGGCGGCGGTGTTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100304 representing YP_002213811
  Red=Cloning sites Green=Tags
  
  MEDINFSSLAPRHGTKPYMGTWSDIGTSQLNGGAFNWSSIWSGLKNFGSTIKTYGNKAWNSSTGQALRNK
  LKDQNFQQKVVDGIASGINGVVDLANQAVQKKINSRLDPPPATPGEMQVEEEIPPPEKRGDKRPRPDLEE
  TLVTRVDEPPSYEEATKLGMPTTRPIAPMATGVMKPSQLHRPVTLDLPPPPAATAVPASKPVAAPKPVAV
  ARSRPGGAPRPNAHWQNTLNSIVGLGVQSVKRRRCF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011202
• ORF Size: 738 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011202.1, YP_002213811
• RefSeq ORF: 738
• Locus ID: 6870505
• MW: 26.6 kDa