E2A (NC_011202) Virus Tagged ORF Clone

CAT#: VC100312

Myc-DDK-tagged ORF clone of viral ORF for single-stranded DNA-binding protein [Human adenovirus B], codon optimized for human cell expression, YP_002213852

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100312 represents NCBI reference of YP_002213852 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCACGCGAAGGCAATCAGCTGTCTGACAGACATAGAGAACATACACCCGAACGGGGAAGGGGCT
  CTGCATCACACCCACCCTCCAGGTCCGATAGGAGTCCTAGCCAGTCTCCGCCCCCTTTGCCCCCCAAACG
  CAATACATGTCGACGAGTAGGTAGTGGAAGCTCCACTGATTCACAGCTGGTAATGGTGAGCGAAACATCA
  CAAAGTAGTCTGTCCCCTGAGAGGTCTGATAGCCCCCCGCCCCCGATCCCTCCTAAGAAGAAGCCGAGGA
  AGACCAAACATATCCCCATGCAAGATATATCCCAGGATTCAGAAGAAGAGCGCGAGGAAGCTCAACTCGT
  GGCCGTCGGGTTCTCATACCCCCCTGTGAGGATTGTGGAAAAGGACGGAAAAAGAAGCATAGAGAAGATC
  GCAAAAGATGACCCTTTGGCCAAGGGCGCCGCCGCTTGCACCGTGAAGAACCCAATTAGCCTGCCTCTCG
  TTAGCGCCTGGGAGAAAGGAATGGAAGTTATGTGCCTTCTGATGGAAAAGTACAGACTGGATAACGAGCT
  CAGGACCTCTTTTAAGCTGATGCCCGAACAACACGAACAGTACAAGCGGATTTGCCATCAGTATGTGAAT
  GAGGAGCATAGGGGAATACAGCTGACCTTTACTTCACATAAGACACTCTCTACCATGATGGGAAGGTTCC
  TGCAGGGAATGATACACTCCTTTTCTCAGATAGCTCATCACAATTGGGAATGTACAGGGTGTGCCTTGTG
  GCCACACGGATGCAACGACTATGAGGGGAAGCTGAAATGCCTTCACGGAAATATCATGATCCAAAAGGAG
  CAGATAATCGAAATGGACGTGGCTTCTGAGAACGGTCAAAGAGCCCTTAAGGAAAATCCCGAACGCACTA
  AAATTACCCAGAACCGCTGGGGTAGATCAGTGGTGCAGATTGCAAATAACGATGCTCGCTGTTGCGTGAA
  CGACGCTGGCTGCGCCGCAAACCAGTTTAGCTCACGCAGCTGTGGAATGTTCTACACGGAGGGCTCCAAG
  GCGCAACAGGCATTCAAGCAGTACGACGCCTTCATGAGAGCCGTGTATCCGGGTATTCGGCAGGACCAAG
  CCAAGATGATACTCATTCCCCTGCACTGTGACTGTAATCATAAGCCTAATTGGGTTCCTGCCATGGGCCG
  GCAGACCTGCAAGATGACACCATTCAGCATCGCAAACGCCGAAGATCTGGACGTCGGGATGATCGCCGAC
  CCTACAGTTTTGGCTTCCGTCCGCCACCCAAGCCTCATGGTGTTTCAGTGTTGCAACCCAGTCTACAGAA
  ACTCCAGAGCTCAGTCCACTGGCCCAAATTGTGATTTTAAGATTTCCGCCCCCGACCTTCTGGGCGCCCT
  GCAGCTGACGAGAAAGTTGTGGTCAGACATCCTCCCAGACATACCCGTTCCGAAGCTCGTTATACCCGAG
  TTTAAATGGCAACCCAAGTACCAGTTCAGAAATGTGAGCCTCCCCGCCGGGCACAGTGATAGTCGCCAGA
  ATCCATTTGACCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100312 representing YP_002213852
  Red=Cloning sites Green=Tags
  
  MASREGNQLSDRHREHTPERGRGSASHPPSRSDRSPSQSPPPLPPKRNTCRRVGSGSSTDSQLVMVSETS
  QSSLSPERSDSPPPPIPPKKKPRKTKHIPMQDISQDSEEEREEAQLVAVGFSYPPVRIVEKDGKRSIEKI
  AKDDPLAKGAAACTVKNPISLPLVSAWEKGMEVMCLLMEKYRLDNELRTSFKLMPEQHEQYKRICHQYVN
  EEHRGIQLTFTSHKTLSTMMGRFLQGMIHSFSQIAHHNWECTGCALWPHGCNDYEGKLKCLHGNIMIQKE
  QIIEMDVASENGQRALKENPERTKITQNRWGRSVVQIANNDARCCVNDAGCAANQFSSRSCGMFYTEGSK
  AQQAFKQYDAFMRAVYPGIRQDQAKMILIPLHCDCNHKPNWVPAMGRQTCKMTPFSIANAEDLDVGMIAD
  PTVLASVRHPSLMVFQCCNPVYRNSRAQSTGPNCDFKISAPDLLGALQLTRKLWSDILPDIPVPKLVIPE
  FKWQPKYQFRNVSLPAGHSDSRQNPFDL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011202
• ORF Size: 1554 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011202.1, YP_002213852
• RefSeq ORF: 1554
• Locus ID: 6870509
• MW: 58.3 kDa