E1A (NC_011203) Virus Tagged ORF Clone

CAT#: VC100328

Myc-DDK-tagged ORF clone of viral ORF for control protein E1A [Human adenovirus B], codon optimized for human cell expression, YP_002213765

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100328 represents NCBI reference of YP_002213765 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGCACCTTAGATTCCTGCCCCAGGAGGTCATCTCCTCAGAAACTGGTATCGAGATCCTGGAGTTTG
  TGGTCAACACCCTGATGGGCGACGACCCCGAGCCTCCCGTGCAGCCTTTCGACCCTCCCACTTTGCATGA
  CCTGTACGACCTTGAAATTGACGGACCGGAAGATCCTAACGAAGAAGCAGTCAATGGATTCTTCACTGAT
  TCAATGCTGCTCGCCGCCGACGAAGGTCTGGACATTAATCCACCTCCCGAAACTCTGGTGACACCCGGAG
  TTGTCGTCGAATCTGGCATCGGCGGTAAGAAGCTGCCCGATCTGGGGGCTGCCGAAATGGATCTGCGATG
  TTATGAAGAAGGGTTTCCTCCCTCCGATGACGAAGACGGAGAAACCGAACAGTCAATCCACACAGCCGTG
  AATGAGGGAGTGAAGGCTGCGTCTGATGTGTTCAAACTGGACTGCCCCGAGCTCCCAGGCCACGGCTGCA
  AGTCTTGCGAGTTCCACAGAAATAATACCGGCATGAAGGAGCTTCTCTGCAGTCTCTGCTATATGAGGAT
  GCACTGCCATTTCATCTATTCCCCCGTGAGCGATGACGAATCCCCTAGCCCGGACAGCACCACATCCCCC
  CCCGAGATTCAGGCACCAGCACCTGCAAATGTGTGTAAGCCCATTCCAGTAAAGCCAAAGCCGGGGAAAC
  GGCCCGCGGTGGACAAGCTGGAGGATCTGCTCGAGGGAGGAGACGGCCCTCTCGATTTGTCCACACGGAA
  ACTGCCCAGGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100328 representing YP_002213765
  Red=Cloning sites Green=Tags
  
  MRHLRFLPQEVISSETGIEILEFVVNTLMGDDPEPPVQPFDPPTLHDLYDLEIDGPEDPNEEAVNGFFTD
  SMLLAADEGLDINPPPETLVTPGVVVESGIGGKKLPDLGAAEMDLRCYEEGFPPSDDEDGETEQSIHTAV
  NEGVKAASDVFKLDCPELPGHGCKSCEFHRNNTGMKELLCSLCYMRMHCHFIYSPVSDDESPSPDSTTSP
  PEIQAPAPANVCKPIPVKPKPGKRPAVDKLEDLLEGGDGPLDLSTRKLPRQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011203
• ORF Size: 783 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011203.1, YP_002213765
• RefSeq ORF: 783
• Locus ID: 6870531
• MW: 28.4 kDa