L2 (NC_011203) Virus Tagged ORF Clone

CAT#: VC100339

Myc-DDK-tagged ORF clone of viral ORF for core protein precursor pVII [Human adenovirus B], codon optimized for human cell expression, YP_002213775

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100339 represents NCBI reference of YP_002213775 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAATACTGATAAGCCCTTCCAATAACACCGGGTGGGGACTCTATGCCCCCACAAAGATGTACGGCG
  GAGCCCGGAAGCGGTCCACTCAACATCCAGTCAGAGTGCGAGGCCACTTCCGCGCACCCTGGGGAGCCCT
  GAAAGGACGAACTCGCACGAGAACAACCGTGGATGACGTGATCGACCAGGTGGTGGCCGACGCACGCAAC
  TACACCCCTACTGCTCCAACATCTACCGTTGACGCCGTGATAGATAGCGTCGTTGCCGACGCCAGAGCTT
  ACGCCCGGCGCAAGTCCAGGCGCAGACGAATTGCCAGGCGACATAGAGCAACCCCCGCTATGAGGGCCGC
  CAAGGCAATACTTCGCCGCGCCAAGCGAGTAGGACGCCGGGCTATGCTGCGAGCAGCAAGAAGGGCGGCT
  TCAGGTGCATCTAGCGGCAGGTCCAGGAGACGCGCCGCCACAGCCGCAGCTGCTGCCATCGCCAATATGG
  CTCAGCCTCGCCGCGGCAATGTGTATTGGGTCAGGGACGCTACCACAGGACAGCGCGTGCCTGTGAGAAC
  TCGGCCCCCCCGGACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100339 representing YP_002213775
  Red=Cloning sites Green=Tags
  
  MSILISPSNNTGWGLYAPTKMYGGARKRSTQHPVRVRGHFRAPWGALKGRTRTRTTVDDVIDQVVADARN
  YTPTAPTSTVDAVIDSVVADARAYARRKSRRRRIARRHRATPAMRAAKAILRRAKRVGRRAMLRAARRAA
  SGASSGRSRRRAATAAAAAIANMAQPRRGNVYWVRDATTGQRVPVRTRPPRT
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011203
• ORF Size: 576 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011203.1, YP_002213775
• RefSeq ORF: 576
• Locus ID: 6870522
• MW: 21.2 kDa