E4 (NC_011203) Virus Tagged ORF Clone

CAT#: VC100362

Myc-DDK-tagged ORF clone of viral ORF for control protein E4 34K [Human adenovirus B], codon optimized for human cell expression, YP_002213836

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100362 represents NCBI reference of YP_002213836 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGGCTCCAACAGCATCATGACTCGACTTAGGGCGAGGTCTACATCTTGTGCTAGGCACCATCCAT
  ATACTAGAGCTCAACTGCCGCGGTGCGAGGAGAACGAAACCAGGGCCTCTATGACCGAAGATCATCCCCT
  TTTGCCCGACTGTGACACCATGACAATGCATAGTGTGAGTTGTGTTCGGGGTCTTCCATGCTCCGCCTCA
  TTCACCGTCCTGCAAGAGTTTCCAATACCTTGGGATATGTTCCTCAACCCCGAAGAGCTGAAGATTATGC
  GCAGGTGTATGCACCTGTGCCTCTGCTGTGCCACGATCGACATCTTCCAGTCACAGGTAATTCACGGGAG
  AGAAAATTGGGTTCTTCACTGCCATTGCAATCAACAGGGGTCATTGCAGTGCATGGCGGGAGGAGCAGTG
  CTGGCTGTTTGGTTCCGAAAGGTTATCTTGGGCTGTATGATTAATCAGCGGTGCCCGTGGTATCGCCAAA
  TAGTGAACATGCATATGCCTAAAGAGATCATGTATGTGGGATCCGTGTTTCTCCGCGAGAGGCACCTGAT
  CTATATAAAACTGTGGTACGATGGCCACGCTGGAGCTATTATTAGCGACATGTCCTTCGGCTGGTCTGCC
  TTTAATTACGGTCTGCTCAACAATATTGTGATTATGTGCTGTACTTACTGTAGCAATCTGTCCGAGATTA
  GGATGCGCTGCTGCGCACATCGGACAAGAAAACTGATGCTTCGCGCCATTAAGATCATGCTGCAAGATAC
  CGCGGATCCCGACCCCATAAACAGCTCCAGGACAGAGCGCCGCCGCCAGCGACTGCTGGTCGGCCTCATG
  CGGCACAATCGGCCGATCCCCTTCAGCGACTACGATTCTCACCGCTCTTCTTCACGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100362 representing YP_002213836
  Red=Cloning sites Green=Tags
  
  MSGSNSIMTRLRARSTSCARHHPYTRAQLPRCEENETRASMTEDHPLLPDCDTMTMHSVSCVRGLPCSAS
  FTVLQEFPIPWDMFLNPEELKIMRRCMHLCLCCATIDIFQSQVIHGRENWVLHCHCNQQGSLQCMAGGAV
  LAVWFRKVILGCMINQRCPWYRQIVNMHMPKEIMYVGSVFLRERHLIYIKLWYDGHAGAIISDMSFGWSA
  FNYGLLNNIVIMCCTYCSNLSEIRMRCCAHRTRKLMLRAIKIMLQDTADPDPINSSRTERRRQRLLVGLM
  RHNRPIPFSDYDSHRSSSR
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011203
• ORF Size: 897 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011203.1, YP_002213836
• RefSeq ORF: 897
• Locus ID: 6870530
• MW: 34.7 kDa