E2B (NC_011203) Virus Tagged ORF Clone

CAT#: VC100335

Myc-DDK-tagged ORF clone of viral ORF for terminal protein precursor pTP [Human adenovirus B], codon optimized for human cell expression, YP_002213832

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100335 represents NCBI reference of YP_002213832 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTGTCAGTTAATGACTGTGCTCGGCTCACAGGCCAGACGGTGCCAACGATGAATTACTTCATGC
  CCCTGCGGAACATCTGGAATCGGGTCAGAGAATTCCCCAGGGCCAGTACTACAGCCTCCGGGATTACTTG
  GATGAGTAGGTACATTTACGGCTACCATCGCCTCATGCTGGAGGACCTGGCCCCCGGAGCGCCCGCGACC
  GAGAGATGGCCTCTTTATCGCCAGCCTCCCCCGCACTTTCTTATCGGTTACCAGTACTTGGTTAGAACCT
  GCAATGATTATATTTTTGATACCCGGGCTTACAGCCGCCTGAAGTACACTGAGCTGGTCAGACCTGGTCA
  TCAGACTGTGAACTGGTCTGTCATGGCAAATTGCTCCTATACCATCAATACCGGAGCCTACCATAGGTTC
  GTGGACTTCGATGATTTTCAAGCAACGCTGACCCAAGTACAACAGGCTATACTGGCTGAGAGGGTAGTTG
  CCGATCTCGCACTTGTGCAGCCTATGCGCGGCTTCGGTATAACGAGGATGCACGGACGAGCGGGGGAGGA
  GGAGGTGCCCGTGGAACGACTCATGCAGGACTACTATAAGGACCTCGCAAGGTGTCAAGACAACGCCTGG
  GGTATGGCGCACCGGCTGCGGATCCAGCAGGCTGGACCGAAGGACCTGGTCCTTCTCGCCACGATCAGAC
  GCCTCAAGACAGCATATTTCAACTTTATCACTAGCTCTATTGTGAGCCCCAGCCAGGAGGAAGAGGGAGA
  GGAGCGAGAAAACCCCGACAGAGCAAGTTCCAGACCTAGACCCCAGGAGACAGTTCTGTCCCTCCCGTGC
  GACTGTGACTGGCTCGATGCCTTTGTGGAACGCTTCAGCGACCCGGTCGACCTTGAAACAATCCGGAGCC
  TTCGAGGAGTACCCACAGGCCAGCTTATAAAGTGTATCATATCCGCAGTTAGCCTGCCAAATGGCGAGCC
  ACCCTCTCATCACTTCCGGGAGATGCGCGGAGGGGTGTTTACCCTGCGGCCTAGAGAAAACGGACGAGCT
  GTTACTGAAACCATGCGACGACGGAGAGGCGAGGTTATCGAGCGATTCATCGACAGGCTGCCAGTTCGCA
  GGAGGCGGCGGCGGGTACCCCCACCCCCTGCGGCTCCGCCTGAGGAGGAGGAAATGCTGGTGGAGGAAGA
  AGAGATAGAGGAAGAGATACCAGGCGCTTTTGAGCGGGAAGTCAGAACTACAATCGCCGAGTTGATTAGA
  CTGCTTGAGGAGGAACTGACAGTGTCCGCCAGGAATTCACAGTTCTTCAACTTTGCGGTAGACTTCTATG
  AAGCGATGGAGAGATTGGAGGCACTGGGGGATGTGTCTGAGATGCCGTTGAGGAGGTGGATAATGTATTT
  CTTTGTTACTGAACATATCGCCACCACCCTTAACTATCTCTTTCAGCGCTTGTGTAATTATGCGGTGTTT
  ACAAGACACGTTGAGCTGAATTTGGCGCAGGTGGTCATGCGGGCCAGAGATCCAGTGGGCGCTGTAGTGT
  ACTCCAGGGTTTGGAACGAGGCCGGGATGAACGCCTTCTCCCAGCTGATCGGTCGCATTTCCAATGACCT
  GGCTGCAACCGTGGAGCGCGCCGGCAGAGGGGATTTGCAAGAGGAGGAAATTGAGCAGTTTATGGCCGAA
  ATAGCTTATCAGGACAATTCAGGCGATGTGCAGGAAATCCTGCGACAAGCCGCCGTGAATGACACTGAAA
  TCGATTCAGTGGAACTCAGCTTCAGGTTCAAACTTACAGGACCCGTCGCCTTCACACAACGGAGACAAAT
  ACAGGACGTGAATAGGCGAGTTGTCGCCCATGCAAGCCTGCTTCGGGCACAATATCAAAATCTGCCTGCT
  AGGGGCGCCGATGTCCCACTCCCTGCAATGCCCCCAGGACCCGAGCCACCTCTTCCTCCTGGAGCAAGAC
  CACGGCACAGATTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100335 representing YP_002213832
  Red=Cloning sites Green=Tags
  
  MALSVNDCARLTGQTVPTMNYFMPLRNIWNRVREFPRASTTASGITWMSRYIYGYHRLMLEDLAPGAPAT
  ERWPLYRQPPPHFLIGYQYLVRTCNDYIFDTRAYSRLKYTELVRPGHQTVNWSVMANCSYTINTGAYHRF
  VDFDDFQATLTQVQQAILAERVVADLALVQPMRGFGITRMHGRAGEEEVPVERLMQDYYKDLARCQDNAW
  GMAHRLRIQQAGPKDLVLLATIRRLKTAYFNFITSSIVSPSQEEEGEERENPDRASSRPRPQETVLSLPC
  DCDWLDAFVERFSDPVDLETIRSLRGVPTGQLIKCIISAVSLPNGEPPSHHFREMRGGVFTLRPRENGRA
  VTETMRRRRGEVIERFIDRLPVRRRRRRVPPPPAAPPEEEEMLVEEEEIEEEIPGAFEREVRTTIAELIR
  LLEEELTVSARNSQFFNFAVDFYEAMERLEALGDVSEMPLRRWIMYFFVTEHIATTLNYLFQRLCNYAVF
  TRHVELNLAQVVMRARDPVGAVVYSRVWNEAGMNAFSQLIGRISNDLAATVERAGRGDLQEEEIEQFMAE
  IAYQDNSGDVQEILRQAAVNDTEIDSVELSFRFKLTGPVAFTQRRQIQDVNRRVVAHASLLRAQYQNLPA
  RGADVPLPAMPPGPEPPLPPGARPRHRF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011203
• ORF Size: 1974 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011203.1, YP_002213832
• RefSeq ORF: 1974
• Locus ID: 6870517
• MW: 75.7 kDa