E3 (NC_011203) Virus Tagged ORF Clone

CAT#: VC100358

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for control protein E3 147K [Human adenovirus B], codon optimized for human cell expression, YP_002213794


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Reconstitution Protocol

USD 400.00

3 Weeks*

Size
    • 10 ug

Product Images

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Symbol E3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100358 represents NCBI reference of YP_002213794 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGGATCCCATTGCAACATCTTCAACAGCCGCCAAGGAACTTCTTGACATGGACGGCAGAGCCAGTG
AACAGCGCCTCATTCAGCTGCGAATCCGCCAGCAGCAGGAGCGAGCAGTGAAAGAACTGCGCGACGCAAT
TGGCATACACCAATGCAAGAAAGGCATTTTTTGTCTGGTGAAGCAATCAAAGATATCTTACGAGATTACC
GCCACCGACCACCGGCTGTCATACGAACTTGGTCCTCAACGCCAGAAGTTCACCTGCATGGTCGGCATCA
ATCCCATCGTCATTACCCAGCAATCAGGGGACACCAAAGGCTGCATTCAGTGTTCATGTGACTCAACAGA
ATGCATCTATACTCTCCTGAAGACACTGTGTGGACTTAGAGACTTGCTGCCTATGAAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100358 representing YP_002213794
Red=Cloning sites Green=Tags

MTDPIATSSTAAKELLDMDGRASEQRLIQLRIRQQQERAVKELRDAIGIHQCKKGIFCLVKQSKISYEIT
ATDHRLSYELGPQRQKFTCMVGINPIVITQQSGDTKGCIQCSCDSTECIYTLLKTLCGLRDLLPMN

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_011203
ORF Size 408 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Reference Data
RefSeq NC_011203.1, YP_002213794
RefSeq ORF 408
Locus ID 6870528
MW 15.3 kDa

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.