L2 (NC_011203) Virus Tagged ORF Clone

CAT#: VC100340

Myc-DDK-tagged ORF clone of viral ORF for core protein V [Human adenovirus B], codon optimized for human cell expression, YP_002213776

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100340 represents NCBI reference of YP_002213776 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAAAAGAAAGTACAAGGAAGAAATGCTGCAGGTTATCGCTCCTGAAATCTATGGCCCACCTGTCA
  AGGATGAGAAGAAGCCAAGAAAAATCAAACGAGTCAAAAACAACAAAAAGGAGGAGGATGATGACGACGG
  ACTGGTGGAGTTCGTGCGGGAATTTGCTCCCAGGCGACGGGTGCAGTGGCGGGGGAGAAAAGTACGGCAG
  GTGCTGAGACCAGGAACTACTGTGGTGTTCACCCCGGGAGAAAGAAGTTCAACTACCTTTAAGAGGTCTT
  ACGATGAGGTGTATGGGGACGATGACATCCTCGAGCAGGCTGCTGATCGACTGGATGAGTTTGCCTATGG
  GAAGCGGACTCGGTCTTCACCGAAAGAAGAGGCAGTGAGCATCCCACTGGACCATGGCAATCCAACTCCA
  TCCTTGAAGCCAGTGACCCTGCAGCAGGTTCTGCCTGTGCCTGCCCGGCGGGGCGTGAAGAGAGAGGGTG
  AAGACCTGTACCCTACCATGCAACTCATGGTGCCTAAACGCCAGAGACTGGAAGACGTCCTGGAAAAGAT
  GAAAGTTGATGCCGACATTCAGCCCGAGGTCAAGGTGCGGCCAATAAAGGAGGTCGCCCCCGGGCTTGGG
  GTGCAGACATTCGACATTAAGATTCCAACTGAGTCAATGGAGGTTCAGACTGAGCCCGCCAAGCCCACTA
  CTTCTATCGAAGTCCAGACGGAACCTTGGATGCCTGCCCCTGTTGCTGCGCCAAGTACAACACGCCGGTC
  CAGGAGAAAGTACGGACCAGCCAGTTTGCTGATGCCTAATTACGCCCTCCACCCCTCAATCATCCCCACA
  CCAGGATATAGGGGAACCAGATACTATCGGAACCGGTCAAATACCAGCCGCAGAAGGAAGCCACCTGCCT
  CTAGGACACGGCGGAGGAGGCGGAGAAACACTGCTAGCAAGCTGACACCCGCAGCGCTCGTGCGGAGGGT
  TTACCGCGACGGCAGAGCCGAGCCACTGACCCTGCCGAGAGCTAGATATCACCCCAGCATTACTACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100340 representing YP_002213776
  Red=Cloning sites Green=Tags
  
  MSKRKYKEEMLQVIAPEIYGPPVKDEKKPRKIKRVKNNKKEEDDDDGLVEFVREFAPRRRVQWRGRKVRQ
  VLRPGTTVVFTPGERSSTTFKRSYDEVYGDDDILEQAADRLDEFAYGKRTRSSPKEEAVSIPLDHGNPTP
  SLKPVTLQQVLPVPARRGVKREGEDLYPTMQLMVPKRQRLEDVLEKMKVDADIQPEVKVRPIKEVAPGLG
  VQTFDIKIPTESMEVQTEPAKPTTSIEVQTEPWMPAPVAAPSTTRRSRRKYGPASLLMPNYALHPSIIPT
  PGYRGTRYYRNRSNTSRRRKPPASRTRRRRRRNTASKLTPAALVRRVYRDGRAEPLTLPRARYHPSITT
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011203
• ORF Size: 1047 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011203.1, YP_002213776
• RefSeq ORF: 1047
• Locus ID: 6870522
• MW: 40.2 kDa