E3 (NC_011203) Virus Tagged ORF Clone

CAT#: VC100352

Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein E3 gp19K [Human adenovirus B], codon optimized for human cell expression, YP_002213788

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100352 represents NCBI reference of YP_002213788 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGCAATTCTCGTAGTCCTGGCCCTGCTCAGCCTGCTGGGCCTTGGCTCAGCCAACCTGAACCCTC
  TGGATCACGATCCTTGCTTGGACTTTGATCCTGAAAACTGTACACTGACCTTTGCTCCAGACACCAGTCG
  ACTGTGTGGGGTCCTGATTAAATGTGGATGGGATTGTAGATCAGTGGAGATTACTCATAACAACAAGACA
  TGGAATAACACTCTGTCCACCACCTGGGAACCTGGTGTTCCGCAATGGTACACCGTGAGTGTCCGGGGAC
  CAGATGGTAGCATTCGAATTTCTAACAACACATTCATCTTCTCCGAAATGTGTGATCTGGCCATGTTTAT
  GAGTAGGCAGTACGATCTGTGGCCCCCAAGCAAAGAGAACATAGTGGCCTTTTCCATAGCGTATTGCCTT
  GTTACTTGTATTATTACAGCCATCATCTGCGTTTGTATACACCTGCTGATCGTCATAAGGCCTCGGCAGT
  CAAATGAGGAGAAGGAGAAGATGCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100352 representing YP_002213788
  Red=Cloning sites Green=Tags
  
  MGAILVVLALLSLLGLGSANLNPLDHDPCLDFDPENCTLTFAPDTSRLCGVLIKCGWDCRSVEITHNNKT
  WNNTLSTTWEPGVPQWYTVSVRGPDGSIRISNNTFIFSEMCDLAMFMSRQYDLWPPSKENIVAFSIAYCL
  VTCIITAIICVCIHLLIVIRPRQSNEEKEKMP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011203
• ORF Size: 516 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011203.1, YP_002213788
• RefSeq ORF: 516
• Locus ID: 6870528
• MW: 19.2 kDa